PNK Treatment of DNA Ends protocol

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Solutions/reagents:

Equipment:

  • Incubator
  • Reaction tubes

Steps:

  1. Reaction Mix (10 µl)
    Use the following table as a checklist for preparing the reaction in reaction tube (1):

     PNK stockT4 ligase buffersubstrate
    Reaction Mix1 µl1 µl8 µl
  2. Reaction Conditions
    Incubate at 37°C for 30 mins.
    Incubate at 65°C for 20 mins.
    Store the tube on ice.

TOTAL TIME REQUIRED FOR THE COMPLETION OF THE PROTOCOL :~ 50 mins

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