(→Map, Sequence and MCS)
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''[[E. coli]]'' [[
''[[E. coli]]'' [[vector]] that creates a C-terminal fusion to an [[intein]] domain, which is useful in techniques such as [[expressed protein ligation]].
Revision as of 17:02, 25 May 2011
Sold by New England Biolabs.
Map, Sequence and MCS
Clone a gene using appropriate restriction sites into the MCS. Protein expressed from this plasmid in E. coli can be purified on a chitin affinity column. The tag can be cleaved on-column with reducing agents such as DTT or MESNA, leaving a C-terminal reactive thioester. Peptides and proteins with N-terminal Cys residues will react with the thioester to form a native peptide bond. This vector is part of the IMPACT-CN kit: