PTXB1

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(References)
Current revision (17:07, 25 May 2011) (view source)
(Map, Sequence and MCS)
 
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==Purpose==
==Purpose==
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''[[E. coli]]'' [[cloning vector]] that creates a C-terminal fusion to an [[intein]] domain, which is useful in techniques such as [[expressed protein ligation]].
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''[[E. coli]]'' cloning [[vectors|vector]] that creates a C-terminal fusion to an [[intein]] domain, which is useful in techniques such as [[expressed protein ligation]].
==Procurement==
==Procurement==
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==Map, Sequence and MCS==
==Map, Sequence and MCS==
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[[Image:PTXB1.svg|600px]]
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*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/Fasta/pTXB1.fsa.txt| Sequence as FASTA]
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*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pTXB1.gbk.txt| Sequence in genbank format]
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*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pTXB1_map.pdf| Technical reference including MCS, map, and other information]
==Use==
==Use==
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[[Category:Material]]
[[Category:Material]]
[[Category:Escherichia coli]]
[[Category:Escherichia coli]]
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[[Category:DNA]]
[[Category:Cloning Vector]]
[[Category:Cloning Vector]]

Current revision

Contents

Purpose

E. coli cloning vector that creates a C-terminal fusion to an intein domain, which is useful in techniques such as expressed protein ligation.

Procurement

Sold by New England Biolabs.

Map, Sequence and MCS

Use

Clone a gene using appropriate restriction sites into the MCS. Protein expressed from this plasmid in E. coli can be purified on a chitin affinity column. The tag can be cleaved on-column with reducing agents such as DTT or MESNA, leaving a C-terminal reactive thioester. Peptides and proteins with N-terminal Cys residues will react with the thioester to form a native peptide bond. This vector is part of the IMPACT-CN kit:

Safety

N/A

References

Contact

Kathryn Muratore

Categories

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