PTXB1: Difference between revisions
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==Map, Sequence and MCS== | ==Map, Sequence and MCS== | ||
[[Image:PTXB1.svg|600px]] | [[Image:PTXB1.svg|600px]] | ||
*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/Fasta/pTXB1.fsa.txt|Sequence as FASTA] | *[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/Fasta/pTXB1.fsa.txt| Sequence as FASTA] | ||
*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pTXB1.gbk.txt|Sequence in genbank format] | *[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/pTXB1.gbk.txt| Sequence in genbank format] | ||
*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pTXB1_map.pdf|Technical reference including MCS, map, and other information] | *[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pTXB1_map.pdf| Technical reference including MCS, map, and other information] | ||
==Use== | ==Use== | ||
Latest revision as of 15:07, 25 May 2011
Purpose
E. coli cloning vector that creates a C-terminal fusion to an intein domain, which is useful in techniques such as expressed protein ligation.
Procurement
Sold by New England Biolabs.
Map, Sequence and MCS
- Sequence as FASTA
- Sequence in genbank format
- Technical reference including MCS, map, and other information
Use
Clone a gene using appropriate restriction sites into the MCS. Protein expressed from this plasmid in E. coli can be purified on a chitin affinity column. The tag can be cleaved on-column with reducing agents such as DTT or MESNA, leaving a C-terminal reactive thioester. Peptides and proteins with N-terminal Cys residues will react with the thioester to form a native peptide bond. This vector is part of the IMPACT-CN kit:
Safety
N/A
