PTXB1

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Contents

Purpose

E. coli cloning vector that creates a C-terminal fusion to an intein domain, which is useful in techniques such as expressed protein ligation.

Procurement

Sold by New England Biolabs.

Map, Sequence and MCS

Use

Clone a gene using appropriate restriction sites into the MCS. Protein expressed from this plasmid in E. coli can be purified on a chitin affinity column. The tag can be cleaved on-column with reducing agents such as DTT or MESNA, leaving a C-terminal reactive thioester. Peptides and proteins with N-terminal Cys residues will react with the thioester to form a native peptide bond. This vector is part of the IMPACT-CN kit:

Safety

N/A

References

Contact

Kathryn Muratore

Categories

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