Pamela K. Kreeger (BE postdoctoral)
Ph.D. Northwestern University, Chemical Engineering, 2005
B.S. Valparaiso University, Chemistry, 2000
First, can cue-signal-response models be developed where the ‘cue’ is an intrinsic part of the cell, such as a genetic mutation? To build such a model, we are utilizing a panel of human colon carcinoma cell lines that have distinct genetic manipulations to both K-Ras and N-Ras. These cell lines show different levels of apoptosis following treatment with TNFalpha, and have differences in both basal and stimulated signaling of key intracellular molecules such as ERK and JNK. Secondly, can cue-signal-response models be developed for more complicated paradigms, especially in vivo models? This project will be done in collaboration with the Jacks lab at MIT, utilizing their genetic mouse models of human cancer. To correlate to our in vitro studies, we will generate a dataset of intracellular signaling and apoptosis in the intestinal epithelium of mice with defined K-Ras and N-Ras genotypes.