Paulsson:Electroporation competent MC1061

Home        Group Meeting        Protocols        Inventory        Journal Watch        Top 100        Links        Facts of Life       

• Electroporation protocol

Batch 11/27/06, labeled with one red dot, made by Per
1.5 x 10(9) cells per ug (transformation efficiency tested with ts-Rep pDNA)
50ul aliquots, some tubes labeled "2x" contain 100ul of cells FINISHED

Batch 01/16/07, labeled with red ink, made by Per
6 x 10(9) cells per ug (transformation efficiency tested with pCR2.1 pDNA)FINISHED
95ul aliquots, can use for trfs.

Batch 03/09/07, labeled "MC1061" with blue ink, 104ul aliquots, made by Per
1 x 10(9) cells per ug (transformation eff. tested w/ 1ng pSilver's Venus, 50ul cells)FINISHED

Batch 06/22/07, labeled "MC" with blue ink, made by Per
1.4 x 10(9) cells per ug (transformation efficiency tested with 100ul cells and 1ng pCR2.1 pDNA)
100 and 200ul aliquotsFINISHED

Batch 07/07, labeled "MC1061" with black ink, ~200ul aliquots, made by Per
use 100 ul per transformation

Batch ~03/08, labeled "MC1061" with red ink, ~200ul aliquots
use ~95 ul per transformation; transformation efficiency ~1.6 x 10(8) cfu per ug plasmid DNA
made by Anna Andersson (using 500ml conical tubes for spins), tested by Per using pCR2.1 (1ng/ul stock)

Batch 06/25/08, labeled "MC1061" with black ink, 100ul aliquots, made by Per
parallel batch prepared using 500ml Corning Centrifuge Tubes (rather than std bottle)
labeled "MC1061" with black ink and one BLUE DOT, 100ul aliquots
Transformation efficiency not yet tested.