Paulsson:Inventory: Difference between revisions

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[[Paulsson:Electroporation competent XL1-Blue | Electroporation competent XL1-Blue]]<br>
[[Paulsson:Electroporation competent XL1-Blue | Electroporation competent XL1-Blue]]<br>
[[Paulsson:Electroporation competent TOP10 | Electroporation competent TOP10]]<br>
[[Paulsson:Electroporation competent TOP10 | Electroporation competent TOP10]]<br>
[[Paulsson:Electroporation competent PMB14 | Electroporation competent T1(MG72)]]<br>
[[Paulsson:Electroporation competent PMB14 | Electroporation competent PMB14]]<br>
[[Paulsson:Electroporation competent PMB27 | Electroporation competent MC1061(L4)]]<br>
[[Paulsson:Electroporation competent PMB27 | Electroporation competent PMB27]]<br>


*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes]
*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes]

Revision as of 14:24, 30 June 2008

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Ordering stuff we don't have

  • email Per a detailed description of what you want (Item, Company, Catalog #, estimated cost)
  • when Per's not around, email your requests to ordering_sysbio@hms.harvard.edu
    • include the following information: vendor / catalog# / Description / estimated price / quantity / unit of measure / grant (if you know) / requestor (you) / lab (Paulsson) / for general or personal use
    • OR for many items from a single company, send a screen-shot of a shopping cart

By Class

Reagents

Strains
Plasmids
DNA reagents
Antibiotics
Fluorescent proteins

Equipment

MicroPulser Electroporation Apparatus (Bio-Rad) manual - Bench #7

By location

Deli Fridge

Antibiotics (Ampicillin, sodium salt; novobiocin; anhydrous tetracycline (inducer); G418)
Chemicals, 4°C (salmon sperm DNA
Qiagen P1 buffer, ECL reagents, glutathione sepharose Solid media

Common bench freezer

Lab Plasmids

Bench #2 freezer

Restriction enzymes
DNA modifying reagents
Chemicals, -20°C (IPTG [1])

Bench #3 fridge

Bench #4 fridge

Bench #5 freezer

Deep freeze (-80°C)

Strains

Strains

Competent Cells

  • Can be found in a box labeled Paulsson Competent Cells on the 2nd shelf, second rack of -80

Please let someone know if our supply is dangerously low (less than 5 tubes left)
Electroporation competent MC1061
Electroporation competent DH5-alpha
Electroporation competent XL1-Blue
Electroporation competent TOP10
Electroporation competent PMB14
Electroporation competent PMB27