Paulsson:Inventory: Difference between revisions
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==Ordering stuff we don't have== | |||
* email Per a detailed description of what you want (Item, Company, Catalog #, estimated cost) | |||
* when Per's not around, email your requests to '''ordering_sysbio@hms.harvard.edu'''<br> | |||
**include the following information: vendor / catalog# / Description / estimated price / quantity / unit of measure / user ("all lab" or your name) / lab (Paulsson)<br> | |||
**'''OR''' for many items from a single company, send a screen-shot of a shopping cart | |||
*April 2009: New purchases no longer entered into FROS database. To search what we've bought in the past, email James... or get access to ____ through HCOM. | |||
*Ordering ethanol: VWR will supply and deliver ethanol to the labs starting Monday, November 1, 2010. The current number to call to order ethanol, 617-432-2298, will automatically call forward to the Harvard Longwood Lab Supply Center’s phone number 617-432-1914. Please let Jane Garfield know if you experience any difficulties during this transition. | |||
==By Class== | ==By Class== | ||
===Reagents=== | ===Reagents=== | ||
| Line 11: | Line 18: | ||
[[Paulsson:Antibiotics | Antibiotics]]<br> | [[Paulsson:Antibiotics | Antibiotics]]<br> | ||
[[Paulsson:Fluorescent proteins | Fluorescent proteins]] | [[Paulsson:Fluorescent proteins | Fluorescent proteins]] | ||
===Equipment=== | |||
MicroPulser Electroporation Apparatus (Bio-Rad) [http://www.bio-rad.com/cmc_upload/Literature/13121/4006174B.pdf manual] - Bench #7<br> | |||
==By location== | ==By location== | ||
===Deli Fridge=== | ===Deli Fridge=== | ||
Antibiotics (Ampicillin, sodium salt; novobiocin; anhydrous tetracycline (inducer); G418)<br> | Antibiotics (Ampicillin, sodium salt; novobiocin; anhydrous tetracycline (inducer); G418)<br> | ||
Chemicals (salmon sperm DNA<br> | Chemicals, 4°C (salmon sperm DNA<br> | ||
Qiagen P1 buffer, ECL reagents, glutathione sepharose | |||
Solid media | |||
===Common bench freezer=== | ===Common bench freezer=== | ||
Lab Plasmids | |||
===Bench #2 freezer=== | ===Bench #2 freezer=== | ||
Restriction enzymes | Restriction enzymes<br> | ||
DNA modifying reagents | DNA modifying reagents<br> | ||
Chemicals (IPTG [http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/i5502pis.pdf])<Br> | Chemicals, -20°C (IPTG [http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/i5502pis.pdf])<Br> | ||
===Bench #3 fridge=== | ===Bench #3 fridge=== | ||
===Deep freeze (- | ===Bench #4 fridge=== | ||
===Bench #5 freezer=== | |||
===Deep freeze (-80°C)=== | |||
*[[Paulsson:-80freezer | freezer map]] | |||
====Strains==== | |||
[[Paulsson:Strains | Strains]]<br> | |||
====Competent Cells==== | ====Competent Cells==== | ||
*Can be found in a box labeled '' | *Can be found in a box labeled ''Competent Cells'' the 2nd shelf, first rack of -80<br> | ||
Please | Please notify if our supply is low (less than ~10 tubes left)<br> | ||
[[Paulsson:Electroporation competent E.coli prepared by Silvia | Electroporation competent E.coli prepared by Silvia ]]<br> | |||
[[Paulsson:Electroporation competent MC1061 | Electroporation competent MC1061]]<br> | [[Paulsson:Electroporation competent MC1061 | Electroporation competent MC1061]]<br> | ||
[[Paulsson:Electroporation competent DH5-alpha | Electroporation competent DH5-alpha]]<br> | [[Paulsson:Electroporation competent DH5-alpha | Electroporation competent DH5-alpha]]<br> | ||
[[Paulsson:Electroporation competent XL1-Blue | Electroporation competent XL1-Blue]]<br> | [[Paulsson:Electroporation competent XL1-Blue | Electroporation competent XL1-Blue]]<br> | ||
[[Paulsson:Electroporation competent TOP10 | Electroporation competent TOP10]]<br> | [[Paulsson:Electroporation competent TOP10 | Electroporation competent TOP10]]<br> | ||
*[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes] | *[http://openwetware.org/wiki/E._coli_genotypes E. coli Genotypes] | ||
===Misc=== | |||
*Abil EM90 (surfactant) | |||
ample free sample (one pint) acquired from company (cosmetic)<br> | |||
Amy Caudill: 804-452-5616 | |||
Latest revision as of 14:43, 13 January 2014
Ordering stuff we don't have
- email Per a detailed description of what you want (Item, Company, Catalog #, estimated cost)
- when Per's not around, email your requests to ordering_sysbio@hms.harvard.edu
- include the following information: vendor / catalog# / Description / estimated price / quantity / unit of measure / user ("all lab" or your name) / lab (Paulsson)
- OR for many items from a single company, send a screen-shot of a shopping cart
- include the following information: vendor / catalog# / Description / estimated price / quantity / unit of measure / user ("all lab" or your name) / lab (Paulsson)
- April 2009: New purchases no longer entered into FROS database. To search what we've bought in the past, email James... or get access to ____ through HCOM.
- Ordering ethanol: VWR will supply and deliver ethanol to the labs starting Monday, November 1, 2010. The current number to call to order ethanol, 617-432-2298, will automatically call forward to the Harvard Longwood Lab Supply Center’s phone number 617-432-1914. Please let Jane Garfield know if you experience any difficulties during this transition.
By Class
Reagents
Strains
Plasmids
DNA reagents
Antibiotics
Fluorescent proteins
Equipment
MicroPulser Electroporation Apparatus (Bio-Rad) manual - Bench #7
By location
Deli Fridge
Antibiotics (Ampicillin, sodium salt; novobiocin; anhydrous tetracycline (inducer); G418)
Chemicals, 4°C (salmon sperm DNA
Qiagen P1 buffer, ECL reagents, glutathione sepharose
Solid media
Common bench freezer
Lab Plasmids
Bench #2 freezer
Restriction enzymes
DNA modifying reagents
Chemicals, -20°C (IPTG [1])
Bench #3 fridge
Bench #4 fridge
Bench #5 freezer
Deep freeze (-80°C)
Strains
Competent Cells
- Can be found in a box labeled Competent Cells the 2nd shelf, first rack of -80
Please notify if our supply is low (less than ~10 tubes left)
Electroporation competent E.coli prepared by Silvia
Electroporation competent MC1061
Electroporation competent DH5-alpha
Electroporation competent XL1-Blue
Electroporation competent TOP10
Misc
- Abil EM90 (surfactant)
ample free sample (one pint) acquired from company (cosmetic)
Amy Caudill: 804-452-5616
