Paulsson:Nanodrop: Difference between revisions
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===Location=== | ===Location=== | ||
=== | ===Access=== | ||
*SysBio people: should have access to the computer and software. If not, they either don't have an ecommons account yet, or their ecommons account has not yet been added to the SysBio group list. Talk to Erin Howard or Mason Miranda. | |||
*Non SysBio people: if they have an ecommons account they can be added to the list of 'collaborators' maintained by Per and Mason. | |||
Here's how (a reminder for Per):<br> | |||
1. Login to ecommons (at any computer)<br> | |||
2. Choose the <Research> tab located below the username near the top of the page<br> | |||
3. In <Applications> child-window choose <Online storage> link<br> | |||
4. At top left of page, select <My Collaborations><br> | |||
5. Select <PAULSSON NanoDrop> folder<br> | |||
6. Select <Members List><br> | |||
7. Search using the last name of the user to be added<br> | |||
Folders and files can also be added to the collaboration folder (e.g. NanoDrop manual, guide for new users) | |||
===Measuring S. pombe cultures=== | ===Measuring S. pombe cultures=== | ||
Revision as of 11:05, 20 August 2008
Nanodrop Spectrophotometer
Location
Access
- SysBio people: should have access to the computer and software. If not, they either don't have an ecommons account yet, or their ecommons account has not yet been added to the SysBio group list. Talk to Erin Howard or Mason Miranda.
- Non SysBio people: if they have an ecommons account they can be added to the list of 'collaborators' maintained by Per and Mason.
Here's how (a reminder for Per):
1. Login to ecommons (at any computer)
2. Choose the <Research> tab located below the username near the top of the page
3. In <Applications> child-window choose <Online storage> link
4. At top left of page, select <My Collaborations>
5. Select <PAULSSON NanoDrop> folder
6. Select <Members List>
7. Search using the last name of the user to be added
Folders and files can also be added to the collaboration folder (e.g. NanoDrop manual, guide for new users)
Measuring S. pombe cultures
Measuring E. coli cultures
It seems that using more than the minimal 2ul can be important for obtaining reliable measurements. 3ul works consistently.
- OD600 = 0.1 is equivalent to approximately 5 x 10(8) cells/ml
Determined at one dilution with MC1061 by counting cells with hemacytometer: OD600 of 0.1 = 4 x 10(8) cells/ml (12/11/06, Per M.)
Determined at one dilution with XL1-BLue by plating cells: OD600 of 0.1 = 5.9 x 10(8) cells/ml (12/19/06, Per M.)
- OD600 measurement linear between values of 0.005 and 0.35
Service/Updates
Thankfully, none yet.
