Paulsson:Protocols: Difference between revisions
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[[Paulsson:Electrocompetent E.coli | Preparation of electrocompetent E.coli]]<br> | [[Paulsson:Electrocompetent E.coli | Preparation of electrocompetent E.coli]]<br> | ||
[[Paulsson:Electroporation of E.coli | Electroporation of E.coli]] | [[Paulsson:Electroporation of E.coli | Electroporation of E.coli]]<Br> | ||
[[Paulsson:Plasmid replication | Measuring DNA replication with 3H-thymidine]]<BR> | [[Paulsson:Plasmid replication | Measuring DNA replication with 3H-thymidine]]<BR> | ||
Revision as of 06:51, 15 August 2007
Many excellent protocols can be found in Current Protocols in Molecular Biology, available through the Countway Digital Library.
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Preparation of electrocompetent E.coli
Electroporation of E.coli
Measuring DNA replication with 3H-thymidine
Getting Started In the Lab
Pipetting 101
general
what volume, which tool
accuracy and calibration (cleaning)
Sterile technique
Nurturining Microbes
preservation growth conditions genotypes antibiotics
Media Preparation
Current Protocols in Molecular Biology article on mimimal, rich, solid and agar media for E. coli
Media:Ecoli_preparation_media.pdf
- MSC media (from Rosenfeld et al., Science v307, 25 March 2005, SOM)
M9 salts
0.6% succinate
0.01% casamino acids
0.15 ug/ml biotin
1.5 uM thiamine
doubling time for E. coli reported to be 45 (-/+10) min at 32 degrees
for microscope pad, dissolve low melt agarose in MSC (1.5%)
Plasmid Biology
definitions transformation isolation segragation incompatability copy number selection
Manipulating DNA
Synthesis
PCR
Enzymes
Quick Change mutagenesis
