Paulsson:Strains
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Paulsson Lab Strain Collection
- Location
- Electronic: Excel file at PAULSSON LAB\STRAINS\Paulsson_Strains
- Physical:
- Boxes: -80°C freezer in , bottom shelf, labeled "JP 1"
- Plates: -80°C freezer in , bottom shelf, labeled "96 A"
- Duplicates: somewhere in the Kishony Lab
- Criteria for inclusion
- Published (always!)
- Sent to another lab (always!)
- Received from another lab (most often, but use your judgement as to general utility)
- Useful (characterized parts; eg. fluorescent proteins, promoters)
- Duplicates in the Kishony Lab freezer
- All published strains
- Precious strains that seem worth it
- The JP collection in the Kishony lab should be maintained in numerical order (discontinuous)
- Guidelines
- For strains carrying plasmids, please add plasmid sequence files to PAULSSON LAB\DNA_sequences (if available)
- Antibiotic conventions: Ap (ampicillin), Kn (kanamycin), Cm (chloramphenicol), Tet (tetracyline), Spc (spectinomycin), Str (streptinomycin), Gm (gentamycin), Rif (rifampicin), Nal (naladixic acid),
Sending and Receiving strains
Strains
Shay's strains
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| ST1 | DH5α | pUC19 | ||
| ST2 | DH5α | pKN1562 | R1, par-, KanR | |
| ST3 | DH5α | pBEU17 | R1, ts runaway | |
| ST4 | MG1655 | ??? | "pAmp-ts CFP (ptet22) in MG1655 yfp (??)" | |
| ST5 | DH5α | pBR322 | ||
| ST6 | DH5α | pTH18kr | ||
| ST7 | DH5α | pTH18ks1 | ||
| ST8 | DH5α | pTH18ks5 | ||
| ST9 | DH5α | pKH19ks1 | ||
| ST10 | DH5α | pTH19ks5 | ||
| ST11 | T1(MGZ2) | TetR, LacI | p(of ST11), Ptet-Citrus | Z2 is Silhavy modified version of Bernard Lutz Z1 repressor insert |
| ST12 | MC1061 | p(of ST12), Ptet-Citrus | ||
| ST13 | MC1061(L4) | TetR, LacI | p(of ST13), Plac-Citrus | |
| ST14 | MC1061 | p(of ST14), Plac-Citrus | ||
| ST15 | MC1061 | pST15 | pTSrep + RNAI | |
| ST51 | CSH50 | pKG339 | ||
| ST52 | MC1061 | pST52 | ||
| ST55 | PMB27 | pST52 / pST54 | ||
| ST61 | CSH50 | pKG339 / pST54 |
Juan's strains
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| JM100 | =MG1655 | Sequenced | ||
| JM101 | MG1655 | DFimH | =PMB 245 | |
| JM102 | MG1655 | DFimH DFlhD | ||
| JM103 | MG1655 | DFimA | =PMB 245 | |
| JM104 | MG1655 | DFimH DFlhD DFlu::Cm | R: Chlm (1) | |
| JM105 | MG1655 | DFimH DFlhD DFlu::Cm DGlgA | R: Chlm (1) |
Per's strains
Box1
| Name | Background | Genotype | Plasmid(s) | Note | |
|---|---|---|---|---|---|
| PMB1 | DH5α | pKS390 | |||
| PMB2 | DH5α | pKS391 | |||
| PMB3 | DH5α | pKS392 | |||
| PMB4 | DH5α | pKS393 | |||
| PMB5 | DH5α | pKS394 | |||
| PMB6 | DH5α | pKS395 | |||
| PMB7 | DH5α | pKS396 | |||
| PMB8 | DH5α | pKS397 | |||
| PMB9 | DH5α | pKS398 | |||
| PMB10 | DH5α | pKS399 | |||
| PMB11 | DH5α | ||||
| PMB12 | MC1061 | from Beckwith/Hochschild Lab | |||
| PMB13 | ?? | "*" cured of plasmid; colonies much smaller than MG1655; MC1061? | |||
| PMB14 | T1(MGZ2) | ST11 cured of plasmid | |||
| PMB15 | ER2925 | from NEB | |||
| PMB16 | DH5-alpha | pSC101-ts | |||
| PMB17 | MC1061 | pSC101-ts | |||
| PMB18 | XL1-Blue | ||||
| PMB19 | TOP10 | ||||
| PMB20 | TOP10 | pCR2.1 w/Citrus-ssrA(#9) | |||
| PMB21 | MC1061 | pPM1 | |||
| PMB22 | MC1061 | pPM2 | |||
| PMB23 | MC1061 | pPM3 | |||
| PMB24 | MC1061 | pPM4 | |||
| PMB25 | MC1061 | pPM5 | |||
| PMB26 | MC1061 | pPM6 | |||
| PMB27 | MC1061(L4) | TetR and LacI | ST13 cured of plasmid | ||
| PMB28 | PMB14 | pPM3 | |||
| PMB29 | PMB14 | pPM5 | |||
| PMB30 | PMB27 | pPM4 | |||
| PMB31 | PMB27 | pPM6 | |||
| PMB32 | MC1061 | pPM7 | |||
| PMB33 | MC1061 | pPM8 | |||
| PMB34 | MC1061 | pPM9 | |||
| PMB35 | MC1061 | pPM10 | |||
| PMB36 | MC1061 | pPM11 | |||
| PMB37 | MC1061 | pPM12 | |||
| PMB38 | MC1061 | pPM13 | |||
| PMB39 | MC1061 | pPM14 | |||
| PMB40 | PMB14 | pPM13 | |||
| PMB41 | PMB27 | pPM14 | |||
| PMB42 | MC1061 | from Kolter Lab | |||
| PMB43 | MC1061 | p(Silver's Venus), from Ian | |||
| PMB44 | MC1061 | pPM15 | |||
| PMB45 | MC1061 | pPM16 | |||
| PMB46 | DH5α | pUB23-R | |||
| PMB47 | DH5α | pUB23-L | |||
| PMB48 | DH5α | pUB23-M | |||
| PMB49 | DH5α | pJT184 (CmR) | |||
| PMB50 | PMB14 | pJT184 (CmR) | |||
| PMB51 | DH5α | pUB23-K | |||
| PMB52 | DH5α | pYPet-His (CmR) | |||
| PMB53 | DH5α | pCyPet-His (CmR) | |||
| PMB54 | PMB14 | pPM15 | |||
| PMB55 | PMB27 | pPM16 | |||
| PMB56 | |||||
| PMB57 | MC1061 | pPM17 | |||
| PMB58 | MC1061 | pPM18 | |||
| PMB59 | MC1061 | pPM19 | |||
| PMB60 | MC1061 | pPM20 |
Box2
| Name | Background | Genotype | Plasmid(s) | Note | |
|---|---|---|---|---|---|
| PMB62 | MC1061 | pPM22 | 240 lacO repeats in pSC101 ts-Rep | ||
| PMB63 | MC1061 | pPM23 | 24 lacO repeats in pSC101 ts-Rep | ||
| PMB64 | MC1061 | pPM24 | 24 tetO repeats in pSC101 ts-Rep | ||
| PMB65 | MC1061 | pPM25 | 120 lacO repeats in pSC101 ts-Rep | ||
| PMB66 | MC1061 | pPM26 | 120 tetO repeats in pSC101 ts-Rep | ||
| PMB67 | MC1061 | pPM27 | LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), forward | ||
| PMB68 | MC1061 | pPM28 | LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), reverse | ||
| PMB69 | MC1061 | pPM29 | Ptet-mCherry in pSC101 | ||
| PMB70 | MC1061 | pPM30 | Plac-mCherry in pSC101 | ||
| PMB74 | PMB14 | pPM29 | |||
| PMB75 | PMB27 | pPM30 | |||
| PMB76 | MC1061 | pPM31 | LacI-YFP in p15A (CmR, and AmpR!?!) | ||
| PMB77 | MC1061 | pPM32 | LacI-CFP, TetR-YFP in p15A (CmR only) | ||
| PMB78 | DH5α | pPM33 | LacI-YFP in p15A (CmR only) | ||
| PMB79 | PMB14 | pPM34 | |||
| PMB80 | PMB14 | pPM35 | |||
| PMB81 | PMB27 | pPM36 | |||
| PMB82 | PMB27 | pPM37 | |||
| PMB83 | PMB14 | pPM38 | Ptet-mCherry in pSC101 ts-Rep | ||
| PMB84 | PMB27 | pPM39 | Plac-mCherry in pSC101 ts-Rep | ||
| PMB85 | MC1061 | pPM40 | |||
| PMB86 | MC1061 | pPM41 | |||
| PMB87 | MC1061 | pPM42 | |||
| PMB89 | ECF259 | pPM32 | |||
| PMB90 | AB1157 | pPM32 | |||
| PMB91 | AB1157 | pPM33 | |||
| PMB92 | AB1157 | pLAU53 | |||
| PMB93 | AB1157 | pPM32/pPM24 | |||
| PMB94 | AB1157 | pPM32/pPM26 | |||
| PMB95 | AB1157 | pPM33/pPM22 | |||
| PMB96 | AB1157 | pPM33/pPM23 | |||
| PMB97 | AB1157 | pPM33/pPM25 | |||
| PMB98 | PMB14 | pPM40 | |||
| PMB99 | PMB14 | pPM41 | |||
| PMB100 | PMB14 | pST26 | same as ST??? | ||
| PMB101 | PMB14 | pST28 | same as ST??? | ||
| PMB102 | MC4100 | galK::Plac-CFP | Hegreness et al., Science '06, via Kishony Lab | ||
| PMB103 | MC4100 | galK::Plac-YFP | Hegreness et al., Science '06, via Kishony Lab | ||
| PMB104 | PMB27 | galK::Plac-CFP | PMB102 transduced into PMB27; not confirmed by PCR, only by CFP expression | ||
| PMB105 | PMB27 | galK::Plac-YFP | PMB103 transduced into PMB27; not confirmed by PCR, only by YFP expression | ||
| PMB106 | PMB104 | pST27 | (Plac-Citrus on R1, par-) | ||
| PMB107 | PMB104 | pPM41 | (Plac-Citrus on mini-F) | ||
| PMB108 | PMB105 | pST29 | (Plac-mCherry on R1, par-) | ||
| PMB110 | PMB27 | pST27 | (Plac-Citrus on R1, par-) | ||
| PMB111 | PMB27 | pST29 | (Plac-mCherry on R1, par-) | ||
| PMB112 | PMB104 | pST26 | (Ptet-Citrus on R1, par-) | ||
| PMB113 | PMB104 | pPM40 | (Ptet-Citrus on mini-F) | ||
| PMB114 | PMB105 | pST28 | (Ptet-mCherry on R1, par-) | ||
| PMB115 | PMB105 | pPM42 | (Ptet-mCherry on mini-F) | ||
| PMB116 | PMB104 | pPM40/pST28 | (Ptet-Citrus on mini-F; Ptet-mCherry on R1,par-) | ||
| PMB117 | PMB104 | pPM40/pST29 | (Ptet-Citrus on mini-F; Plac-mCherry on R1,par-) | ||
| PMB118 | PMB107 | pPM41/pST28 | (Plac-Citrus on mini-F; Ptet-mCherry on R1,par-) | ||
| PMB119 | PMB107 | pPM41/pST29 | (Plac-Citrus on mini-F; Plac-mCherry on R1,par-) | ||
| PMB120 | PMB14 | pPM42 | (Ptet-mCherry on mini-F) | ||
| PMB121 | MC1061 | pPM43 | |||
| PMB122 | MC1061 | pPM44 | |||
| PMB123 | MC1061 | pPM45 | |||
| PMB124 | MC1061 | pPM46 | |||
| PMB125 | PMB14 | pPM43 | (Ptet-YPet on pSC101, ts-Rep) | ||
| PMB126 | PMB27 | pPM44 | (Plac-YPet on pSC101, ts-Rep) | ||
| PMB127 | PMB14 | pPM45 | (Ptet-YPet on pSC101) | ||
| PMB128 | PMB27 | pPM46 | (Plac-YPet on pSC101) | ||
| PMB129 | PMB14 | pST26/pPM30 | |||
| PMB130 | PMB27 | pST26/pPM30 | (Ptet-Citrus on R1, par-; Ptet-mCherry on pSC101) | ||
| PMB131 | PMB14 | pPM11 | |||
| PMB132 | PMB27 | pPM12 |
Box3
| Name | Background | Genotype | Plasmid(s) | Note | |
|---|---|---|---|---|---|
| PMB133 | PMB14 | pST26/pPM42 | |||
| PMB134 | ST51 | pST26 | ST51 is CSH50/pKG339 (Plac-copA from Gerdes) | ||
| PMB135 | ST51 | pST28 | |||
| PMB136 | PMB14 | pST26/pKG339 | |||
| PMB137 | PMB112 | pKG339 | |||
| PMB138 | PMB114 | pKG339 | |||
| PMB139 | ST52 | pST26 | |||
| PMB140 | ST50 | pKG339 | |||
| PMB141 | ST51 | pKG491 | |||
| PMB142 | ST51 | pKN1562 | |||
| PMB143 | ST51 | pST27 | |||
| PMB144 | PMB12 | pST52 | |||
| PMB146 | PMB27 | pST52 | |||
| PMB147 | PMB27 | pST52/pKN1562 | |||
| PMB148 | PMB27 | pKG339 | |||
| PMB149 | PMB27 | pKG339/pST26 | |||
| PMB150 | PMB27 | pKG339/pKG491 | |||
| CSH50 | "CHS50" generated by curing plasmid from ST51 | ||||
| PMB151 | CSH50 | pST52 | |||
| PMB152 | CSH50 | pST52/pKN1562 | |||
| PMB153 | CSH50 | pKG339 | |||
| PMB154 | CSH50 | pKG339/pKN1562 | |||
| PMB155*** | CSH50 | pKG339/pKG491 | ***(may be bad?!AmpR/IPTG-res subpopulation) | ||
| PMB156 | PMB27 | pST26 | |||
| PMB157 | PMB27 | pKG491 | |||
| PMB158 | MC1061 | pPM47 | |||
| PMB159 | MC1061 | pPM48 | |||
| PMB160 | MC1061 | attTn7::Ptet-mCherry | |||
| PMB161 | MC1061 | attTn7::Plac-mCherry | |||
| PMB162 | PMB27 | attTn7::Ptet-mCherry | |||
| PMB163 | PMB27 | attTn7::Plac-mCherry | |||
| PMB164 | PMB14 | attTn7::Ptet-mCherry | |||
| PMB165 | PMB27 | attTn7::Ptet-mCherry | pST26 | ||
| PMB166 | PMB27 | attTn7::Plac-mCherry | pST27 | ||
| PMB167 | PMB14 | attTn7::Ptet-mCherry | pST26 | ||
| PMB168 | PMB27 | attTn7::Ptet-mCherry | pST26/pST52 | ||
| PMB169 | PMB27 | attTn7::Plac-mCherry | pST27/pST52 | ||
| PMB170 | PMB14 | attTn7::Ptet-mCherry | pST26/pST52 | ||
| PMB171 | MC1061 | pKGCm | |||
| PMB173 | CSH50 | pKGCm | |||
| PMB175 | MC4100 | pKGCm | |||
| PMB177 | AB1157 | pKG339 | |||
| PMB178 | AB1157 | pKG339/pKG491 | |||
| PMB179 | MC4100 | pKG339 | |||
| PMB180 | MC4100 | pKG339/pKG491 | |||
| PMB181 | MG1655 | pKG339 | |||
| PMB182 | MG1655 | pKG339/pKG491 | |||
| PMB183 | W3110 | pKG339 | |||
| PMB184 | W3110 | pKG339/pKG491 | |||
| PMB185 | MC1061 | forthcoming | pPM49 | ||
| PMB186 | MC1061 | pPM50 | |||
| PMB187 | MC1061 | pPM51 | |||
| PMB188 | MC1061 | pPM52 | |||
| PMB189 | MC1061 | pPM53 | |||
| PMB190 | MC1061 | pPM54 |
Box4
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB191 | CSH50 | pPM54 | ||
| PMB192 | DJ480 | pKG339 | ||
| PMB193 | DJ480 | pPM54 | ||
| PMB194 | CSH50 | pKG339/pPM50 | ||
| PMB195 | CSH50 | pKG339/pPM51 | ||
| PMB196 | CSH50 | pKG339/pPM52 | ||
| PMB197 | CSH50 | pPM54/pKG491 | ||
| PMB198 | DJ480 | pKG339/pKG491 | ||
| PMB199 | DJ480 | pPM54/pPM50 | ||
| PMB200 | DJ480 | pPM54/pPM51 | ||
| PMB201 | DJ480 | pPM54/pPM52 | ||
| PMB202 | DJ480 | pPM54/pKG491 | ||
| PMB203 | MC1061 | pPM55 | ||
| PMB204 | MC1061 | pPM56 | clone 1 (also preserved PMB204 with clone 3) | |
| PMB205 | MC1061 | pPM57 | ||
| PMB206 | MC1061 | pPM58 | ||
| PMB207 | MC1061 | pPM59 | ||
| PMB208 | PM27 | pPM60 | ||
| PMB209 | PM27 | pPM61 | ||
| PMB210 | PM27 | pPM62 | ||
| PMB211 | PM27 | pPM63 | ||
| PMB212 | PM27 | pPM64 | ||
| PMB213 | PM27 | pPM65 | ||
| PMB214 | PM27 | pPM66 | ||
| PMB215 | PM27 | pPM67 | ||
| PMB216 | MC1061 | pPM68 | ||
| PMB217 | DJ480 | pPM54/pPM59 | ||
| PMB218 | PMB27 | pPM70 | ||
| PMB219 | PMB27 | pPM71 | ||
| PMB220 | PMB27 | pPM72 | ||
| PMB221 | PMB27 | pPM73 | ||
| PMB222 | PMB27 | pPM74 | ||
| PMB223 | CSH50 (Gerdes) | ΔfimA::kan | P1 trans. from Keio KO collection | |
| PMB224 | CSH50 (CGSC 8085) | ΔfimA::kan | P1 trans. from Keio KO collection | |
| PMB225 | MG1655seq (CGSC 7740) | ΔfimE::kan | P1 trans. from Keio KO collection | |
| PMB226 | MG1655seq (CGSC 7740) | ΔthiC::kan | P1 trans. from Keio KO collection | |
| PMB227 | PMB223 | pKG491 | ||
| PMB228 | CGSC 8085 | pKG491 | ||
| PMB229 | PMB224 | pKG491 | ||
| PMB230 | PMB225 | pKG491 | ||
| PMB231 | PMB226 | pKG491 | ||
| PMB232 | CGSC 6412 | pKG491 | ||
| PMB233 | PMB223 | pKG491/pKG339 | ||
| PMB234 | CGSC 8085 | pKG491/pKG339 | ||
| PMB235 | PMB224 | pKG491/pKG339 | ||
| PMB236 | PMB225 | pKG491/pKG339 | ||
| PMB237 | PMB226 | pKG491/pKG339 | ||
| PMB238 | CGSC 6412 | pKG491/pKG339 | ||
| PMB239 | CSH50 (KG) | ΔfimA | made by P1 from Keio, marker removed | |
| PMB240 | CSH50 (KG) | ΔfimA | pPM54 | made by P1 from Keio, marker removed |
| PMB241 | CSH50 (CGSC# 8085) | ΔfimA | made by P1 from Keio, marker removed | |
| PMB242 | CSH50 (CGSC# 8085) | ΔfimA | pPM54 | |
| PMB243 | CSH50 (CGSC# 8085) | pPM54 | ||
| PMB244 | MG1655(seq) | ΔfimA | made by P1 from Keio, marker removed | |
| PMB245 | MG1655(seq) | ΔfimH | made by P1 from Keio, marker removed | |
| PMB246 | CSH50 (KG) | ΔfimH | made by P1 from Keio, marker removed | |
| PMB247 | CSH50 (KG) | DfimH | pPM54 | |
| PMB248 | ZK4 | recA56 | pPM54 | MC4100 background |
| PMB249 | ZK356 | recA1 | pPM54 | MC1000 background |
| PMB250 | MC1061 | pPM75 | ||
| PMB251 | MC1061 | pPM76 | ||
| PMB252 | MC1061 | pPM77 | ||
| PMB253 | CGSC# 7740 | pKG491 | ||
| PMB254 | DJ480 | pKG491 | ||
| PMB255 | PMB239 | pPM54/pKG491 | ||
| PMB256 | PMB246 | pPM54/pKG491 | ||
| PMB257 | MC1061 | pPM78 | clone 1 | |
| PMB258 | MC1061 | pPM79 | clone 3' | |
| PMB259 | MC1061 | pPM80-3 | ||
| PMB260 | MC1061 | pPM82 | clone 1 | |
| PMB261 | MC1061 | pPM83 | clone 1 (also preserved PMB261 with clone 2) | |
| PMB262 | MC1061 | pPM84 | clone 3 | |
| PMB263 | MC1061 | pPM85 | clone 2 | |
| PMB264 | MC1061 | pPM86 | ||
| PMB265 | MC1061 | pPM87 | ||
| PMB266 | MC1061 | pPM80-2 | ||
| PMB267 | MC1061 | pPM90 | clone 4 | |
| PMB268 | MC1061 | pPM88 | clone 2 | |
| PMB269 | MC1061 | pPM89 | clone 1 | |
| PMB270 | MC1061 | pPM88 | clone 1 |
Box5
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB271 | CSH50(KG) | pKG491/pPM82 | ||
| PMB272 | CSH50(KG) | pKG491/pPM75 | ||
| PMB273 | CSH50(KG) | pKG491/pPM83 | ||
| PMB274 | DJ480 | pKG491/pPM82 | ||
| PMB275 | DJ480 | pKG491/pPM75 | ||
| PMB276 | DJ480 | pKG491/pPM83 | ||
| PMB277 | CGSC#7740 | pKG491/pPM54 | ||
| PMB278 | CGSC#7740 | pKG491/pPM82 | ||
| PMB279 | CGSC#7740 | pKG491/pPM75 | ||
| PMB280 | CGSC#7740 | pKG491/pPM83 | ||
| PMB281 | CSH50 | pPM78 | ||
| PMB282 | DJ480 | pPM78 | ||
| PMB283 | CSH50 | pPM77 | ||
| PMB284 | DJ480 | pPM77 | ||
| PMB285 | CSH50 | pPM86 | ||
| PMB286 | DJ480 | pPM86 | ||
| PMB287 | CSH50 | pPM87 | ||
| PMB288 | DJ480 | pPM87 | ||
| PMB289 | CSH50 | pPM88-1 | ||
| PMB290 | DJ480 | pPM88-1 | ||
| PMB291 | CSH50 | pPM89 | ||
| PMB292 | DJ480 | pPM89 | ||
| PMB293 | MC1061 | pPM80-5 | ||
| PMB294 | MC1061 | pPM80-6 | ||
| PMB295 | MC1061 | pPM81 | clone 4 | |
| PMB296 | MC1061 | pPM91 | ||
| PMB297 | MC1061 | pPM92 | ||
| PMB298 | MC1061 | pPM93 | ||
| PMB299 | MC1061 | pPM94 | ||
| PMB300 | MC1061 | pPM95 | ||
| PMB301 | MC1061 | pPM96 | ||
| PMB302 | MC1061 | pPM97 | ||
| PMB303 | MC1061 | pPM98 | ||
| PMB304 | PMB239 | pPM82 | ||
| PMB305 | PMB239 | pPM75 | ||
| PMB306 | PMB239 | pPM83 | ||
| PMB307 | PMB239 | pKG491 | ||
| PMB308 | PMB246 | pPM82 | ||
| PMB309 | PMB246 | pKG491 | ||
| PMB310 | DJ480 | pPM83 | ||
| PMB311 | DJ480 | pKGCm | ||
| PMB312 | PMB239 | pPM54/pKG491 | (made from PMB240) | |
| PMB313 | PMB239 | pPM82/pKG491 | (made from PMB304) | |
| PMB314 | PMB239 | pPM82/pPM91 | (made from PMB304) | |
| PMB315 | PMB239 | pPM82/pPM93 | (made from PMB304) | |
| PMB316 | PMB239 | pPM82/pPM94 | (made from PMB304) | |
| PMB317 | PMB239 | pPM82/pPM97 | (made from PMB304) | |
| PMB318 | PMB239 | pPM82/pPM98 | (made from PMB304) | |
| PMB319 | PMB239 | pPM75/pKG491 | (made from PMB305) | |
| PMB320 | PMB239 | pPM83/pKG491 | (made from PMB306) | |
| PMB321 | PMB239 | pKG491/pPM83 | (made from PMB307) | |
| PMB322 | PMB239 | pKG491/pKGCm | (made from PMB307) | |
| PMB323 | DJ480 | pKGCm/pKG491 | (made from PMB311) | |
| PMB324 | MC1061 | pPM99 | ||
| PMB325 | MC1061 | pPM100 | ||
| PMB326 | MC1061 | pPM101 | ||
| PMB327 | MC1061 | pPM102 | ||
| PMB328 | MC1061 | pPM103A | ||
| PMB329 | MC1061 | pPM103B | ||
| PMB330 | MC1061 | pPM106A | ||
| PMB331 | MC1061 | pPM107A | ||
| PMB332 | MC1061 | pPM110A | ||
| PMB333 | MC1061 | pPM110B | ||
| PMB334 | MC1061 | pPM111A | ||
| PMB335 | DJ480 | pKG491/pPM106A | made from PMB254 | |
| PMB336 | DJ480 | pKG491/pPM107A | made from PMB254 | |
| PMB337 | DJ480 | pKG491/pPM110A | made from PMB254 | |
| PMB338 | DJ480 | pKG491/pPM110B | made from PMB254 | |
| PMB339 | DJ480 | pKG491/pPM111A | made from PMB254 | |
| PMB340 | CSH50 | pKG491/pPM106A | made from ST50 | |
| PMB341 | CSH50 | pKG491/pPM107A | made from ST50 | |
| PMB342 | CSH50 | pKG491/pPM110A | made from ST50 | |
| PMB343 | CSH50 | pKG491/pPM110B | made from ST50 | |
| PMB344 | CSH50 | pKG491/pPM111A | made from ST50 | |
| PMB345 | CSH50 | pKG491/pPM54/pKGCm | made from PMB197 | |
| PMB346 | DJ480 | pKG491/pPM54/pKGCm | made from PMB202 | |
| PMB347 | PMB246 | pKG491/pPM54/pKGCm | made from PMB256 |
Box 6
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB348 | MC1061 | pPM93T-1 | ||
| PMB349 | MC1061 | pPM93T-2 | ||
| PMB350 | MC1061 | pPM95T-2 | ||
| PMB351 | MC1061 | pPM95T-3 | ||
| PMB352 | MC1061 | pPM99T-1 | ||
| PMB353 | MC1061 | pPM99T-2 | ||
| PMB354 | MC1061 | pPM101T-1 | ||
| PMB355 | MC1061 | pPM101T-2 | ||
| PMB357 | DJ480 | pPM54/pPM90 | made from PMB193 | |
| PMB358 | DJ480 | pPM54/pPM95T-2 | made from PMB193 | |
| PMB359 | DJ480 | pPM54/pPM95T-3 | made from PMB193 | |
| PMB360 | DJ480 | pPM54/pPM101T-1 | made from PMB193 | |
| PMB361 | CSH50 DfimH | pPM54/pPM81 | made from PMB247 | |
| PMB362 | CSH50 DfimH | pPM54/pPM93-1 | made from PMB247 | |
| PMB363 | CSH50 DfimH | pPM54/pPM93T-2 | made from PMB247 | |
| PMB364 | CSH50 DfimH | pPM54/pPM94 | made from PMB247 | |
| PMB365 | CSH50 DfimH | pPM54/pPM91-3 | made from PMB247 | |
| PMB366 | CSH50 DfimH | pPM54/pPM99 | made from PMB247 | |
| PMB367 | CSH50 DfimH | pPM54/pPM99T-1 | made from PMB247 | |
| PMB368 | CSH50 DfimH | pPM54/pPM99T-2 | made from PMB247 | |
| PMB369 | CSH50 DfimH | pPM54/pPM100 | made from PMB247 | |
| PMB370 | CSH50 DfimH | pKG491/pPM106A | made from PMB309 | |
| PMB371 | CSH50 DfimH | pKG491/pPM107A | made from PMB309 | |
| PMB372 | MC1061 | pPM112 | ||
| PMB373 | MC1061 | pPM113 | ||
| PMB374 | DJ480 | pPM82 | ||
| PMB375 | DJ480 | pPM106A | ||
| PMB376 | DJ480 | pPM107A | ||
| PMB377 | MC4100 | pPM54 | ||
| PMB378 | MC4100 | pPM82 | ||
| PMB379 | MC4100 | pPM106A | ||
| PMB380 | MC1061 | pPM114 | clone 1 | |
| PMB381 | MC1061 | pPM115 | clone 1 | |
| PMB382 | MC1061 | pPM95T-7 | ||
| PMB383 | MC1061 | pPM95T-8 | ||
| PMB384 | MC1061 | pPM116 | clone 1 | |
| PMB385 | MC1061 | pPM117 | clone 1 | |
| PMB386 | MC1061 | pPM118 | clone 1 | |
| PMB387 | MC1061 | pPM119 | clone 1 | |
| PMB388 | MC1061 | pPM120 | clone 1 | |
| PMB389 | MC1061 | pPM121* | clone 1 | |
| PMB390 | MC1061 | pPM122 | clone 2 | |
| PMB391 | MC1061 | pPM123 | clone 3 | |
| PMB392 | MC1061 | pPM124 | clone 1 | |
| PMB393 | MC1061 | pPM125 | clone 1 | |
| PMB394 | MC1061 | pPM126 | clone 2 | |
| PMB395 | MC1061 | pPM127 | clone 3 | |
| PMB396 | MC1061 | pPM130 | clone 1 | |
| PMB397 | MC1061 | pPM131 | clone 1 | |
| PMB398 | MC1061 | pPM132 | clone 1 | |
| PMB399 | MC1061 | pPM133 | clone 1 | |
| PMB400 | MC1061 | pPM134 | clone 1 | |
| PMB401 | DJ480 | pPM54/pPM81 | made from PMB193 | |
| PMB402 | DJ480 | pPM54/pPM90 | made from PMB193 | |
| PMB403 | DJ480 | pPM54/pPM112 | made from PMB193 | |
| PMB404 | DJ480 | pPM54/pPM113 | made from PMB193 | |
| PMB405 | DJ480 | pPM54/pPM122 | made from PMB193 | |
| PMB406 | DJ480 | pPM54/pPM123 | made from PMB193 | |
| PMB407 | DJ480 | pPM54/pPM124 | made from PMB193 | |
| PMB408 | DJ480 | pPM54/pPM125 | made from PMB193 | |
| PMB409 | DJ480 | pPM54/pPM126 | made from PMB193 | |
| PMB410 | DJ480 | pPM54/pPM127 | made from PMB193 | |
| PMB411 | DJ480 | pPM54/pPM131 | made from PMB193 | |
| PMB412 | DJ480 | pPM54/pPM132 | made from PMB193 | |
| PMB413 | DJ480 | pPM54/pPM133 | made from PMB193 | |
| PMB414 | DJ480 | pPM54/pPM134 | made from PMB193 | |
| PMB415 | MC1061 | pPM135 | clone 1 | |
| PMB416 | MC1061 | pPM136 | clone 2 | |
| PMB417 | MC1061 | pPM137 | clone 1 | |
| PMB418 | MC1061 | pPM138 | clone 1 | |
| PMB419 | MC1061 | pPM139 | clone 1 | |
| PMB420 | MC1061 | pPM140 | clone 9 | |
| PMB421 | MC1061 | pPM141 | clone 2 | |
| PMB422 | MC1061 | pKG491 | ||
| PMB423 | MC1061 | pKN1562 | ||
| PMB424 | DJ480 | pPM54/pACT7 | made from PMB193 |
Box 7
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB425 | DJ480 | pPM54/pPM135 | made from PMB193 | |
| PMB426 | DJ480 | pPM54/pPM136 | made from PMB193 | |
| PMB427 | DJ480 | pPM54/pPM137 | made from PMB193 | |
| PMB428 | DJ480 | pPM54/pPM138 | made from PMB193 | |
| PMB429 | DJ480 | pPM54/pPM139 | made from PMB193 | |
| PMB430 | DJ480 | pPM54/pPM140 | made from PMB193 | |
| PMB431 | DJ480 | pPM54/pPM141 | made from PMB193 | |
| PMB432 | MC1061 | pPM91-3 | ||
| PMB433 | DJ480 | pPM106A/pPM90 | made from PMB375 | |
| PMB434 | DJ480 | pPM106A/pPM113 | made from PMB375 | |
| PMB435 | DJ480 | pPM106A/pPM135 | made from PMB375 | |
| PMB436 | DJ480 | pPM106A/pPM136 | made from PMB375 | |
| PMB437 | DJ480 | pPM106A/pPM137 | made from PMB375 | |
| PMB438 | DJ480 | pPM106A/pPM138 | made from PMB375 | |
| PMB439 | DJ480 | pPM106A/pPM139 | made from PMB375 | |
| PMB440 | DJ480 | pPM106A/pPM140 | made from PMB375 | |
| PMB441 | DJ480 | pPM106A/pPM141 | made from PMB375 | |
| PMB442 | DJ480 | pPM106A/pPM122 | made from PMB375 | |
| PMB443 | DJ480 | pPM106A/pPM123 | made from PMB375 | |
| PMB444 | DJ480 | pPM106A/pACT7 | made from PMB375 | |
| PMB445 | DJ480 | pPM54/pACT7/pPM135 | made from PMB424 | |
| PMB446 | DJ480 | pPM54/pACT7/pPM136 | made from PMB424 | |
| PMB447 | DJ480 | pPM54/pACT7/pPM140 | made from PMB424 | |
| PMB448 | DJ480 | attTn7::PLtet-mCherry | made using pPM47 for integration | |
| PMB449 | MG1655 | attTn7::PLtet-mCherry | made using pPM47 for integration | |
| PMB450 | MC4100 | attTn7::PLtet-mCherry | made using pPM47 for integration | |
| PMB451 | DJ480 | pPM106A/pPM137 | made from PMB375; remake of PMB437 | |
| PMB452 | DJ480 | pPM107A/pPM137 | made from PMB376 | |
| PMB453 | DJ480 | attTn7::PLtet-mCherry | pPM54 | made from PMB448 |
| PMB454 | DJ480 | attTn7::PLtet-mCherry | pPM106A | made from PMB448 |
| PMB455 | DJ480 | pPM54/pPM142 (clone 7) | made from PMB193 | |
| PMB456 | DJ480 | pUA66 | ||
| PMB457 | DJ480 | pUA66/pPM136 | ||
| PMB458 | DJ480 | pUA66/pPM137 | ||
| PMB459 | DJ480 | pUA66/pPM139 | ||
| PMB460 | DJ480 | pPM136 | ||
| PMB461 | DJ480 | pPM137 | ||
| PMB462 | DJ480 | pT7POL23 | ||
| PMB463 | DJ480 | pPM136/pT7POL23 | made from PMB460 | |
| PMB464 | DJ480 | pPM136/pT7POL26 | made from PMB460 | |
| PMB465 | MC4100 | pPM54/pPM136 | made from PMB377 | |
| PMB466 | MC4100 | pPM54/pPM137 | made from PMB377 | |
| PMB467 | MC4100 | pPM54/pPM139 | made from PMB377 | |
| PMB468 | MC4100 | pPM54/pPM140 | made from PMB377 | |
| PMB469 | MC4100 | pPM54/pPM141 | made from PMB377 | |
| PMB470 | MC4100 | pPM54/pPM142 | made from PMB377 | |
| PMB471 | DJ480 | araB::T7RNAP_tetA | pPM54/pPM136 | made from PMB479 |
| PMB472 | MC4100 | araB::T7RNAP_tetA | pPM54/pPM136 | made from PMB480 |
| PMB473 | DJ480 | araB::T7RNAP_tetA | P1 from BL21-AI to DJ480 | |
| PMB474 | MC4100 | araB::T7RNAP_tetA | P1 from BL21-AI to MC4100 | |
| PMB475 | DJ480 | araB::T7RNAP_tetA | pPM54/pPM140 | made from PMB479 |
| PMB476 | MC4100 | araB::T7RNAP_tetA | pPM54/pPM140 | made from PMB480 |
| PMB477 | DJ480 | araB::T7RNAP_tetA | pPM136 | made from PMB473 |
| PMB478 | MC4100 | araB::T7RNAP_tetA | pPM136 | made from PBM474 |
| PMB479 | DJ480 | araB::T7RNAP_tetA | pPM54 | made from PMB473 |
| PMB480 | MC4100 | araB::T7RNAP_tetA | pPM54 | made from PBM474 |
| PMB481 | MC4100 | araB::T7RNAP_tetA | pPM54/pPM136/pLysS | made from PBM472 |
| PMB482 | DJ480 | attP::tetR & lacIq | DJ480 transduced with P1 from PMB14 | |
| PMB483 | DJ480 | ΔacrA, attP::tetR & lacIq | ST88 transduced with P1 from PMB14 | |
| PMB484 | DJ480 | attP::tetR & lacIq | pPM54 | trf of PMB482 |
| PMB485 | DJ480 | ΔacrA, attP::tetR & lacIq | pPM54 | trf of PMB483 |
| PMB486 | DJ480 | attP::tetR & lacIq | pPM54/pPM93 | trf of PMB484 |
| PMB487 | DJ480 | attP::tetR & lacIq | pPM54/pPM95 | trf of PMB484 |
| PMB488 | DJ480 | ΔacrA, attP::tetR & lacIq | pPM54/pPM93 | trf of PMB485 |
| PMB489 | DJ480 | ΔacrA, attP::tetR & lacIq | pPM54/pPM95 | trf of PMB485 |
| PMB490 | TOP10 | pCP20 | from Dirk (Datsenko & Wanner) | |
| PMB491 | BW27783 | araB::T7RNAP_tetA | P1 from PMB14 into BW27783 from Raul (nalR?) | |
| PMB492 | BW27783 | araB::T7RNAP_tetA | pPM54 | made from PMB491 |
| PMB493 | BW27783 | araB::T7RNAP_tetA | pPM54/pPM136 | made from PMB492 |
| PMB494 | BW27783 | araB::T7RNAP_tetA | pPM54/pPM140 | made from PMB492 |
Box 8
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB495 | MC1061 | pPM144 | clone 3 | |
| PMB496 | MC1061 | pPM145 | clone 1 | |
| PMB497 | MC1061 | pPM146 | clone 1 | |
| PMB498 | MC1061 | pPM147 | clone 2 | |
| PMB499 | MC1061 | pPM148 | clone 1 | |
| PMB500 | MC1061 | pPM149 | clone 1 | |
| PMB501 | MC1061 | pPM150 | clone 1 | |
| PMB502 | MC1061 | pPM151 | clone 1 | |
| PMB503 | MC1061 | pPM152 | clone 1 | |
| PMB504 | MC1061 | pPM153 | clone 1 | |
| PMB505 | MC1061 | pPM154 | clone 3 | |
| PMB506 | MC1061 | pPM155 | clone 1 | |
| PMB507 | MC1061 | pPM156 | clone 1 | |
| PMB508 | MC1061 | pPM157 | forthcoming | |
| PMB509 | MC1061 | pPM158 | clone 1 | |
| PMB510 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM144 | |
| PMB511 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM148 | |
| PMB512 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM152 | |
| PMB513 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM153 | |
| PMB514 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM156 | |
| PMB515 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM158 | |
| PMB516 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM144 | |
| PMB517 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM148 | |
| PMB518 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM152 | |
| PMB519 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM153 | |
| PMB520 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM156 | |
| PMB521 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM158 | |
| PMB522 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM156/pM152 | made from PMB514; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml) |
| PMB523 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM156/pM152 | made from PMB520; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml) |
| PMB524 | MC1061 | pPM160 | clone 1 | |
| PMB525 | MC1061 | pPM161 | clone 1 | |
| PMB526 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM160 | |
| PMB527 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM161 | |
| PMB528 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM160 | |
| PMB529 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM161 | |
| PMB530 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM160/pPM153 | |
| PMB531 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM161/pPM152 | pSC101_Plac-copA_Kn / R1(-)_Pt7-gfpmut3_Cm / R1(-)_Pt7-mCherry_Ap |
| PMB532 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM160/pPM153 | |
| PMB533 | PMB492 | BW27783 araB::T7RNAP_tetA | pPM54/pPM161/pPM152 | |
| PMB535 | MC1061 | pPM145 A5 | clone A+5; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25 | |
| PMB536 | MC1061 | pPM145 A6 | clone A+6; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25 | |
| PMB537 | MC1061 | pPM162 | clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25 | |
| PMB538 | MC1061 | pPM163 | clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25 | |
| PMB539 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM160-1 | redo of PMB526. Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn) |
| PMB540 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM160-2 | Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn) |
| PMB541 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM145 (A5) | selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25 |
| PMB542 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM162-1 | selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25 |
| PMB543 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM163-1(1) | clone-1, selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25 |
| PMB544 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM163-1(2) | clone-2(small), selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25 |
| PMB545 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM145 A5-2 | selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20 |
| PMB546 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM163-1a | selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20; not the same as PMB543=479/163-1(1) |
| PMB547 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM163-1b | selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20 |
| PMB548 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM163-1c | selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20 |
| PMB549 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM145 (A5) | 145A5 plasmid prep from PMB535 grown in Cb20; trfs selected on Ap10; glycerol stock cultured in LBglucose/Cb10 (forgot Kn) |
| PMB550 | MC1061 | pPM164 (2) - no good | clone 2; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50 | |
| PMB551 | MC1061 | pPM164 (3) - no good | clone 3; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50 | |
| PMB552 | MC1061 | pPM165 (4) | clone 4; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50 | |
| PMB553 | MC1061 | pPM165 (5) - no good | clone 5; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50 | |
| PMB554 | MC1061 | pPM166 | clone 25-6; trfs selected on LB/Ap25; glycerol stock cultured in LB/Cb10 | |
| PMB555 | MC1061 | pPM167 | clone 80-6; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10 | |
| PMB556 | MC1061 | pPM168 | clone 80-7; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10 | |
| PMB557 | MC1061 | pPM169 | clone T1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB558 | MC1061 | pPM170 | clone T2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB559 | MC1061 | pPM171 | clone T3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB560 | MC1061 | pPM172 | clone E1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB561 | MC1061 | pPM173 | clone E2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB562 | MC1061 | pPM174 | clone E3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20 | |
| PMB563 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM165 | trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20 |
| PMB564 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM169 | trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20 |
| PMB565 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM170 | trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20 |
| PMB566 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM171 | trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20 |
| PMB567 | MC1061 | pPM175 | trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25 | |
| PMB568 | MC1061 | pPM176 | trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25 | |
| PMB569 | MC1061 | pPM177 | trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25 | |
| PMB570 | MC1061 | pPM178 | trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25 | |
| PMB571 | MC1061 | pPM179 | trfs selected on LB/Ap25; restruck on LB/Ap20; glycerol stock cultured in LB/Ap25 | |
| PMB572 | PMB479 | DJ480 araB::T7RNAP_tetA | pPM54/pPM179 | selected on LBgluc(0.2%)/Kn35/Ap20, grown at 30°C; glycerol stock cultured in LB/Cb25 |
Box 9
| Name | Background | Genotype | Plasmid(s) | Note |
|---|---|---|---|---|
| PMB573 | MG1655 | DJ480 araB::T7RNAP_tetA | pKGCm | made from PMB473; selected on LBgluc(0.2%)/Cm15 |
| PMB574 | MG1655 | DJ480 araB::T7RNAP_tetA | pPM136/pKGCm | made from PMB477; selected on LBgluc(0.2%)/Ap40/Cm15 |
| PMB575 | MG1655 | DJ480 araB::T7RNAP_tetA | pPM54/pKGCm | made from PMB479; selected on LBgluc(0.2%)/Kn35/Cm8 |
| PMB576 | MG1655 | DJ480 araB::T7RNAP_tetA | pPM54/pPM136/pKGCm | made from PMB471; selected on LBgluc(0.2%)/Kn35/Cb32/Cm4 |
| PMB577 | MG1655 | DJ480 araB::T7RNAP_tetA | pPM54/pPM170/pKGCm | made from PMB565; selected on LBgluc(0.2%)/Kn35/Cb32/Cm4 |
| PMB578 | MC1061 | pPM180 | clone 1 | |
| PMB579 | MC1061 | pPM181 | clone 1 | |
| PMB580 | MC1061 | pAM238 | SpcR | |
| PMB581 | MC1061 | pNG162 | SpcR | |
| PMB582 | MC1061 | pNG162a | SpcR | |
| PMB583 | MC1061 | pKG339 | TetR | |
| PMB584 | MC1061 | pPM182 | SpcR; clone 1-1 | |
| PMB585 | MC1061 | pPM183 | SpcR; clone 2-1 | |
| PMB586 | MC1061 | pPM184 | SpcR; clone 5-1 | |
| PMB587 | MC1061 | pPM185 | SpcR; clone 6-1 | |
| PMB588 | PMB473 | pPM182 | SpcR; clone 1-1 | |
| PMB589 | PMB473 | pPM183 | SpcR; clone 2-1 | |
| PMB590 | PMB473 | pKGCm/pPM184 | SpcR; clone 5-1 | |
| PMB591 | PMB473 | pKGCm/pPM185 | SpcR; clone 6-1 | |
| PMB592 | MC1061 | pPM186 | SpcR; clone A-1 | |
| PMB593 | MC1061 | pPM187 | SpcR; clone B-1 | |
| PMB594 | PMB473 | pPM186 | ||
| PMB595 | PMB473 | pPM187 | ||
| PMB596 | PMB473 | pPM136/pPM182 | made from PMB477 | |
| PMB597 | PMB473 | pPM136/pPM183 | made from PMB477 | |
| PMB598 | PMB473 | pPM136/pPM186 | made from PMB477 | |
| PMB599 | PMB473 | pPM136/pPM187 | made from PMB477 | |
| PMB600 | PMB473 | pKGCm/pPM186 | made from PMB573 | |
| PMB601 | PMB473 | pKGCm/pPM187 | made from PMB573 | |
| PMB602 | PMB473 | pKGCm/pPM136/pPM184 | made from PMB574 | |
| PMB603 | PMB473 | pKGCm/pPM136/pPM185 | made from PMB574 | |
| PMB604 | PMB473 | pKGCm/pPM136/pPM186 | made from PMB574 | |
| PMB605 | PMB473 | pKGCm/pPM136/pPM187 | made from PMB574 |
Nate's Strains
| Name | Background | Genotype | Plasmid(s) | Note | Notebook Page | |
|---|---|---|---|---|---|---|
| NDL-1 | One Shot 2T1R (Invitrogen) | ccdB survival cells. Recovered from pGRG37 by curing. | L.2.14 | |||
| NDL-2 | MC4100 | Lac- | pNDL-17, Alon 2-A-6 | e.9.2 | ||
| NDL-3 | MC4100 | Lac- | pNDL-17, Alon 11-A-6 | E.9.2 | ||
| NDL-4 | MC4100 | Lac- | pNDL-17, Alon 13-B-9 | E.9.2 | ||
| NDL-5 | MC4100 | Lac- | pNDL-17, Alon 18-E-2 | E.9.2 | ||
| NDL-6 | MG1655 | pNDL-18 | Promoterless GFP, +CFP, -term | L.6.14 | ||
| NDL-7 | MG1655 | pNDL-19 | Promoterless GFP,-CFP, +term | L.6.14 | ||
| NDL-8 | MG1655 | pNDL-20 | promoterless GFP,+term,+CFP | L.6.14 | ||
| NDL-9 | MG1655 | attTn7::(no promoter)-GFPmut2 | Generated by curing NDL-6 of pNDL-18 | L.6.28 | ||
| NDL-10 | MG1655 | attTN7::B0015-(no promoter)-GFPmut2 | Generated by curing NDL-7 of pNDL-19 | L.6.28 | ||
| NDL-11 | MG1655 | attTn7::B0015-(no promoter)-GFPmut2 | Generated by curing NDL-8 of pNDL-20 | L.6.28 | ||
| NDL-12 | MG1655 | pNDL-21 | Bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-13 | MG1655 | pNDL-22 | Bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-14 | MG1655 | pNDL-23 | very bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-15 | MG1655 | pNDL-24 | very bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-16 | MG1655 | pNDL-25 | Intermediate bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-17 | MG1655 | pNDL-26 | Intermediate bright ColE1 RNA1-tdCherry on R1 | L.7.19 | ||
| NDL-18 | MG1655 | pNDL-31 | Bright ColE1 RNA1-tdCherry in pNDL6 integration vector | L.11.11 | ||
| NDL-19 | MG1655 | pNDL-32 | Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vecotr | L.11.11 | ||
| NDL-20 | MG1655 | pNDL-33 | Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vector | L.11.11 | ||
| NDL-21 | MG1655 | attTn7::pRNA1(bright)-tdCherry | none | bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-31) | L.11.19 | |
| NDL-22 | MG1655 | attTn7::pRNA1(very bright)-tdCherry | none | Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-32) | L.11.19 | |
| NDL-23 | MG1655 | attTn7::pRNA1(very bright)-tdCherry | none | Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-33) | L.11.19 | |
| NDL-24 | MG1655 | pNDL-41 | Contains plasmid with FRT-flanked KanR gene. For Integrations. | |||
| NDL-25 | DH5-Alpha | 'WT' | none | Stock for cloning strain-- plain DH5 alpha cells. | ||
| NDL-26 | BL21-AI (Invitrogen) | pNDL-44 | Contains plasmid with T7 His-bt-B-gal expression construct | 39.25 | ||
| NDL-27 | MG1655 | pNDL-46 | Strain for integration of 1-E-3 Alon Clone | L.17.1 | ||
| NLD-28 | MG1655 | pNDL-47 | Strain for Integration of 1-E-5 Alon Clone | L.17.1 | ||
| NDL-29 | MG1655 | pNDL-48 | Strain for Integration of 2-A-6 Alon Clone | L.17.1 | ||
| NDL-30 | MG1655 | pNDL-49 | Strain for Integration of 2-C-11 Alon Clone | L.17.1 | ||
| NDL-31 | MG1655 | pNDL-50 | Strain for Integration of 2-F-4 Alon Clone | L.17.1 | ||
| NDL-32 | MG1655 | none | NDL-27 cured of pNDL-46. Alon clone integrant. | L.17.2 | ||
| NDL-33 | MG1655 | none | NDL-28 cured of pNDL-47. Alon clone integrant. | L.17.2 | ||
| NDL-34 | MG1655 | none | NDL-29 cured of pNDL-48. Alon clone integrant. | L.17.2 | ||
| NDL-35 | MG1655 | none | NDL-30 cured of pNDL-49. Alon clone integrant. | L.17.2 | ||
| NDL-36 | MG1655 | none | NDL-31 cured of pNDL-50. Alon clone integrant. | L.17.2 | ||
| NDL-37 | BL-21 | lacY1 mutation (Novagen says its a LacZY mutation) | none | LacZ- strain for protein expression. Used in purificaton of btB-gal | 42.1 | |
| NDL-38 | NDL-37 (Novagen Tuner DE3) | lacY1 mutation (Novagen says its a LacZY mutation) | pNDL-44 | For purification of btB-gal | 42.1 | |
| NLD-39 | MG1655 | Wanner strain for integration into chromosome | pKD46 (pNDL-???) | |||
| NDL-40 | MG1655 | pNDL-52 pRNA1-mCherry integrated downstream of attTn7 | cured of pKD46 | Constitutive mCherry strain for library, prior to P1 transduction | L.18.6 | |
| NDL-41 | BL-21 AI | Contains Ara-Inducible T7 Polymerase | pNDL-96 | For expression of GST-cotA | 43.9 | |
| NDL-42 | Rosetta2 DE3 (Novagen) | BL21 derived Strain from Novagen with codon+ plasmid | pRARE2 | For recombinant protein expression with rare codons | L.47.1 | |
| NDL-43 | MG1655 (CGSC 7740) | Fully sequenced MG1655 Strain | none | Host strain for library construction | L.18.15 | |
| NDL-44 | Tuner BL21 (NDL-37) | BL21 (DE3) lacZY mutant | pRARE2 (camR) | Codon+ Tuner Strain | ex.48.2 | |
| NDL-45 | MG1655 | near attTn7::pBR322 pRNA1-mCherry-FRT-KanR | None | Product of P1 from NDL-40 into MG1655 (NDL-43) | L.18.15 | |
| NDL-46 | Tuner BL21 (NDL-37) | BL21 (DE3) LacZY mutant | pRARE2 (camR), pNDL-44 (ampR) | Strain for expression of His-bt-B-gal | 48.3 | |
| NDL-47 | MG1655 | near attTn7::pRNA1-tdCherry | none | FLP-removed KanR from NDL-45 | L.18.9 | |
| NDL-48 | BW25113 | ΔMotB::KanR | none | Keio ΔMotB strain CGSC 9564 | L.19.1 | |
| NDL-49 | BW25113 | ΔMotA::KanR | none | Keio ΔMotA strain CGSC 9564 | L.19.1 | |
| NDL-50 | BW25113 | ΔFliC::KanR | none | Keio ΔfliC strain CGSC 9586 | L.19.1 | |
| NDL-51 | NDL-47(-3) | ΔMotB::kanR near attTn7::pRNA1-mCherry | none | NDL-47 with keio 9564 deletion | L.19.5 | |
| NDL-52 | NDL-47(-3) | ΔMotA::kanR near attTn7::pRNA1-mCherry | none | NDL-47 with keio 9565 deletion | L.19.5 | |
| NDL-53 | NDL-47(-3) | ΔfliC::kanR near attTN7::pRNA1-mCherry | none | NDL-47 with keio 9586 deletion | L.19.5 | |
| NDL-54 | MG1655 | ΔMotA::kanR | none (pKD46 cured) | MG1655 with keio 9565 deletion | L.20.5 | |
| NDL-55 | MG1655 | ΔMotB::kanR | none (pKD46 cured) | MG1655 with keio 9564 deletion | L.20.5 | |
| NDL-56 | NDL-47 | ΔMotA::kanR near attTn7::pRNA1-mCherry | none | P1vir:NDL-54 -> NDL-47 | L.20.7 | |
| NDL-57 | NDL-47 | ΔmotB::kanR near attTn7::pRNA1-mCherry | none | p1vir:NDL-55 -> NDL-47 | L.20.7 | |
| NDL-58 | NDL-47 | ΔmotA near attTn7::pRNA1-mCherry | none | NDL-56 following FLP removal of KanR | L.20.11 | |
| NDL-59 | NDL-47 | ΔmotB near attTn7::pRNA1-mCherry | none | NDL-57 following FLP removal of KanR | L.20.11 | |
| NDL-93 | MG1655 | ΔmotA | none | NDL-54 following FLP removal of KanR | L.24.6 | |
| NDL-94 | MG1655 | ΔmotB | none | NDL-55 following FLP removal of KanR | L.24.6 | |
| NDL-95 | BW25113 | ΔlacI::KanR | none | KanR lacI Keio strain | T.2.1 | |
| NDL-96 | MG1655 | ΔlacI::KanR | none | P1vir-NDL-95 ---> MG1655 | T.2.1 | |
| NDL-97 | MG1655 | DE3 (specR) | none | P1vir-PMB14----> MG1655 | T.2.1 | |
| NDL-98 | BL21 | DE3 | pNDL-99 (pGK8-GFP003} | T.3.1 | ||
| NDL-99 | BL21 | DE3 | pNDL-100 (pGK8-GFP008} | T.3.1 | ||
| NDL-100 | BL21 | DE3 | pNDL-101 (pGK8-GFP012} | T.3.1 | ||
| NDL-101 | BL21 | DE3 | pNDL-102 (pGK8-GFP021} | T.3.1 | ||
| NDL-102 | BL21 | DE3 | pNDL-103 (pGK8-GFP037} | T.3.1 | ||
| NDL-103 | BL21 | DE3 | pNDL-104 (pGK8-GFP038} | T.3.1 | ||
| NDL-104 | BL21 | DE3 | pNDL-105 (pGK8-GFP053} | T.3.1 | ||
| NDL-105 | BL21 | DE3 | pNDL-106 (pGK8-GFP100} | T.3.1 | ||
| NDL-106 | BL21 | DE3 | pNDL-107 (pGK8-GFP170} | T.3.1 | ||
| NDL-107 | BL21 | DE3 | pNDL-108 (pGK8-GFP182} | T.3.1 | ||
| NDL-108 | TB10 | Wanner Strain with Heat Inducible Machinery | none | L.something | ||
| NDL-109 | CP78 (CGSC 4695) | thr,leu,his,arg auxotroph (relA WT) | none | T.5.1 | ||
| NDL-110 | CP79 (CGSC 4696) | thr,leu,his,arg auxotroph (relA2-- null) | none | T.5.1 | ||
| NDL-111 | BW25113 (CGSC 10159) | Keio RelA knockout (kanR) | none | T.5.1 | ||
| NDL-153 | CP78 (NDL-109,CGSC 4695) | thr,leu,his,arg auxotroph (RelA WT) | pKD46 | Strain for Wanner Integration of GFP Reporters | T.2.2 | |
| NDL-154 | CP79 (NDL-110,CGSC 4696) | thr,leu,his,arg auxotroph (relA2--null) | pKD46 | Strain for Wanner Integration of GFP Reporters | T.2.2 | |
| NDL-155 | TB10 | heat inducible labmda red prophage | none | pRNA-1/mCherry-FRT-kanR integrant | L.25.23 | |
| NDL-156 | TB10 | heat inducible labmda red prophage | none | pRNA-1/mCherry/mKate2 hybrid-FRT-kanR integrant | L.25.23 | |
| NDL-157 | TB10 | heat inducible labmda red prophage | none | pRNA-1/mKate2-FRT-kanR integrant | L.25.23 | |
| NDL-158 | NDL-93 | Δ motA | none | P1vir_NDL-155--> NDL-93 | L.25.23 | |
| NDL-159 | NDL-93 | Δ motA | none | P1vir_NDL-156--> NDL-93 | L.25.23 | |
| NDL-160 | NDL-93 | Δ motA | none | P1vir_NDL-157--> NDL-93 | L.25.23 | |
| NDL-161 | NDL-93 | Δ motA near attTN7 pRNA1-mCherry | none | FLP removed kanR from NDL-158 | L.25.31 | |
| NDL-162 | NDL-93 | Δ motA near attTN7 pRNA1-mCherry-mKate2 hybrid | none | FLP removed kanR from NDL-159 | L.25.31 | |
| NDL-162 | NDL-93 | Δ motA near attTN7 pRNA1-mKate2 | none | FLP removed kanR from NDL-160 | L.25.31 | |
| NDL-192 | TB10 | Xie lon-Venus fusion | none | From Sunney Xie Library | ||
| NDL-193 | TB10 | Xie AtpD-Venus fusion | none | From Sunney Xie Library | ||
| NDL-194 | TB10 | Xie lig-Venus fusion | none | From Sunney Xie Library | ||
| NDL-195 | TB10 | Xie GapA-Venus fusion | none | From Sunney Xie Library | ||
| NDL-196 | B. Subtilis | TMN-690 (pYqxm-mCherry, pHag-GFP) | none | from Tom Norman | ||
| NDL-197 | B. Subtilis | TMN-694 (pYqxm-mCherry, pHag-GFP, ΔslrR) | none | from Tom Norman | ||
| NDL-198 | B. Subtilis | TMN- | none | from Tom Norman | ||
| NDL-199 | MG1655 (CGSC 7740) | WT | none | Obtained from CGSC | L.27.1 | |
| NDL-200 | MG1655 (CGSC 6300) | WT | none | Obtained from CGSC (3 colonies saved) | L.27.1 | |
| NDL-201 | MG1655 (CGSC 6300) | near attTn7::pRNA1-mCherry-mKate2-kanR Hybrid | none | Obtained from transduction of NDL-200 with p1Vir.NDL-156 | L.27.2 | |
| NDL-202 | MG1655 (CGSC 6300) | near attTn7::pRNA1-mCherry-mKate2 hybrid | none | obtained from NDL-201 by FLP-removal of kanR marker | L.27.4 | |
| NDL-203 | MG1655 (CGSC 6300) | near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA-kanR | none | p1Vir.NDL-54--> NDL-202 | L.27.7 | |
| NDL-204 | MG1655 (CGSC 6300) | near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA | none | obtained by FLP removal of kanR marker from NDL-203. NOTE: may still have pCP20. See L.27.8 | L.27.8 | |
| NDL-205 | NDL-162 | near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library AtpD-Venus | none | P1vir.NDL-192--> NDL-162 | L.29.2 | |
| NDL-206 | NDL-162 | near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library GapA-Venus | none | P1vir.NDL-193--> NDL-162 | L.29.2 | |
| NDL-207 | NDL-162 | near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library LigA-Venus | none | P1vir.NDL-194--> NDL-162 | L.29.2 | |
| NDL-208 | NDL-162 | near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library Lon-Venus | none | P1vir.NDL-195--> NDL-162 | L.29.2 |
