Paulsson:Strains

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Contents

Paulsson Lab Strain Collection

  • Location
  1. Electronic: Excel file at PAULSSON LAB\STRAINS\Paulsson_Strains
  2. Physical:
      • Boxes: -80°C freezer in , bottom shelf, labeled "JP 1"
      • Plates: -80°C freezer in , bottom shelf, labeled "96 A"
      • Duplicates: somewhere in the Kishony Lab
  • Criteria for inclusion
  1. Published (always!)
  2. Sent to another lab (always!)
  3. Received from another lab (most often, but use your judgement as to general utility)
  4. Useful (characterized parts; eg. fluorescent proteins, promoters)
  • Duplicates in the Kishony Lab freezer
  1. All published strains
  2. Precious strains that seem worth it
  3. The JP collection in the Kishony lab should be maintained in numerical order (discontinuous)
  • Guidelines
  1. For strains carrying plasmids, please add plasmid sequence files to PAULSSON LAB\DNA_sequences (if available)
  2. Antibiotic conventions: Ap (ampicillin), Kn (kanamycin), Cm (chloramphenicol), Tet (tetracyline), Spc (spectinomycin), Str (streptinomycin), Gm (gentamycin), Rif (rifampicin), Nal (naladixic acid),

Sending and Receiving strains

Sending and Receiving strains

Strains


Shay's strains

Name Background Genotype Plasmid(s) Note
ST1 DH5α pUC19
ST2 DH5α pKN1562 R1, par-, KanR
ST3 DH5α pBEU17 R1, ts runaway
ST4 MG1655 ??? "pAmp-ts CFP (ptet22) in MG1655 yfp (??)"
ST5 DH5α pBR322
ST6 DH5α pTH18kr
ST7 DH5α pTH18ks1
ST8 DH5α pTH18ks5
ST9 DH5α pKH19ks1
ST10 DH5α pTH19ks5
ST11 T1(MGZ2) TetR, LacI p(of ST11), Ptet-Citrus Z2 is Silhavy modified version of Bernard Lutz Z1 repressor insert
ST12 MC1061 p(of ST12), Ptet-Citrus
ST13 MC1061(L4) TetR, LacI p(of ST13), Plac-Citrus
ST14 MC1061 p(of ST14), Plac-Citrus
ST15 MC1061 pST15 pTSrep + RNAI
ST51 CSH50 pKG339
ST52 MC1061 pST52
ST55 PMB27 pST52 / pST54
ST61 CSH50 pKG339 / pST54

Juan's strains

Name Background Genotype Plasmid(s) Note
JM100 =MG1655 Sequenced
JM101 MG1655 DFimH =PMB 245
JM102 MG1655 DFimH DFlhD
JM103 MG1655 DFimA =PMB 245
JM104 MG1655 DFimH DFlhD DFlu::Cm R: Chlm (1)
JM105 MG1655 DFimH DFlhD DFlu::Cm DGlgA R: Chlm (1)

Per's strains

Box1

Name Background Genotype Plasmid(s) Note
PMB1 DH5α pKS390
PMB2 DH5α pKS391
PMB3 DH5α pKS392
PMB4 DH5α pKS393
PMB5 DH5α pKS394
PMB6 DH5α pKS395
PMB7 DH5α pKS396
PMB8 DH5α pKS397
PMB9 DH5α pKS398
PMB10 DH5α pKS399
PMB11 DH5α
PMB12 MC1061 from Beckwith/Hochschild Lab
PMB13 ?? "*" cured of plasmid; colonies much smaller than MG1655; MC1061?
PMB14 T1(MGZ2) ST11 cured of plasmid
PMB15 ER2925 from NEB
PMB16 DH5-alpha pSC101-ts
PMB17 MC1061 pSC101-ts
PMB18 XL1-Blue
PMB19 TOP10
PMB20 TOP10 pCR2.1 w/Citrus-ssrA(#9)
PMB21 MC1061 pPM1
PMB22 MC1061 pPM2
PMB23 MC1061 pPM3
PMB24 MC1061 pPM4
PMB25 MC1061 pPM5
PMB26 MC1061 pPM6
PMB27 MC1061(L4) TetR and LacI ST13 cured of plasmid
PMB28 PMB14 pPM3
PMB29 PMB14 pPM5
PMB30 PMB27 pPM4
PMB31 PMB27 pPM6
PMB32 MC1061 pPM7
PMB33 MC1061 pPM8
PMB34 MC1061 pPM9
PMB35 MC1061 pPM10
PMB36 MC1061 pPM11
PMB37 MC1061 pPM12
PMB38 MC1061 pPM13
PMB39 MC1061 pPM14
PMB40 PMB14 pPM13
PMB41 PMB27 pPM14
PMB42 MC1061 from Kolter Lab
PMB43 MC1061 p(Silver's Venus), from Ian
PMB44 MC1061 pPM15
PMB45 MC1061 pPM16
PMB46 DH5α pUB23-R
PMB47 DH5α pUB23-L
PMB48 DH5α pUB23-M
PMB49 DH5α pJT184 (CmR)
PMB50 PMB14 pJT184 (CmR)
PMB51 DH5α pUB23-K
PMB52 DH5α pYPet-His (CmR)
PMB53 DH5α pCyPet-His (CmR)
PMB54 PMB14 pPM15
PMB55 PMB27 pPM16
PMB56
PMB57 MC1061 pPM17
PMB58 MC1061 pPM18
PMB59 MC1061 pPM19
PMB60 MC1061 pPM20

Box2

Name Background Genotype Plasmid(s) Note
PMB62 MC1061 pPM22 240 lacO repeats in pSC101 ts-Rep
PMB63 MC1061 pPM23 24 lacO repeats in pSC101 ts-Rep
PMB64 MC1061 pPM24 24 tetO repeats in pSC101 ts-Rep
PMB65 MC1061 pPM25 120 lacO repeats in pSC101 ts-Rep
PMB66 MC1061 pPM26 120 tetO repeats in pSC101 ts-Rep
PMB67 MC1061 pPM27 LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), forward
PMB68 MC1061 pPM28 LacI-CFP, TetR-YFP in p15A (CmR, and AmpR!?!), reverse
PMB69 MC1061 pPM29 Ptet-mCherry in pSC101
PMB70 MC1061 pPM30 Plac-mCherry in pSC101
PMB74 PMB14 pPM29
PMB75 PMB27 pPM30
PMB76 MC1061 pPM31 LacI-YFP in p15A (CmR, and AmpR!?!)
PMB77 MC1061 pPM32 LacI-CFP, TetR-YFP in p15A (CmR only)
PMB78 DH5α pPM33 LacI-YFP in p15A (CmR only)
PMB79 PMB14 pPM34
PMB80 PMB14 pPM35
PMB81 PMB27 pPM36
PMB82 PMB27 pPM37
PMB83 PMB14 pPM38 Ptet-mCherry in pSC101 ts-Rep
PMB84 PMB27 pPM39 Plac-mCherry in pSC101 ts-Rep
PMB85 MC1061 pPM40
PMB86 MC1061 pPM41
PMB87 MC1061 pPM42
PMB89 ECF259 pPM32
PMB90 AB1157 pPM32
PMB91 AB1157 pPM33
PMB92 AB1157 pLAU53
PMB93 AB1157 pPM32/pPM24
PMB94 AB1157 pPM32/pPM26
PMB95 AB1157 pPM33/pPM22
PMB96 AB1157 pPM33/pPM23
PMB97 AB1157 pPM33/pPM25
PMB98 PMB14 pPM40
PMB99 PMB14 pPM41
PMB100 PMB14 pST26 same as ST???
PMB101 PMB14 pST28 same as ST???
PMB102 MC4100 galK::Plac-CFP Hegreness et al., Science '06, via Kishony Lab
PMB103 MC4100 galK::Plac-YFP Hegreness et al., Science '06, via Kishony Lab
PMB104 PMB27 galK::Plac-CFP PMB102 transduced into PMB27; not confirmed by PCR, only by CFP expression
PMB105 PMB27 galK::Plac-YFP PMB103 transduced into PMB27; not confirmed by PCR, only by YFP expression
PMB106 PMB104 pST27 (Plac-Citrus on R1, par-)
PMB107 PMB104 pPM41 (Plac-Citrus on mini-F)
PMB108 PMB105 pST29 (Plac-mCherry on R1, par-)
PMB110 PMB27 pST27 (Plac-Citrus on R1, par-)
PMB111 PMB27 pST29 (Plac-mCherry on R1, par-)
PMB112 PMB104 pST26 (Ptet-Citrus on R1, par-)
PMB113 PMB104 pPM40 (Ptet-Citrus on mini-F)
PMB114 PMB105 pST28 (Ptet-mCherry on R1, par-)
PMB115 PMB105 pPM42 (Ptet-mCherry on mini-F)
PMB116 PMB104 pPM40/pST28 (Ptet-Citrus on mini-F; Ptet-mCherry on R1,par-)
PMB117 PMB104 pPM40/pST29 (Ptet-Citrus on mini-F; Plac-mCherry on R1,par-)
PMB118 PMB107 pPM41/pST28 (Plac-Citrus on mini-F; Ptet-mCherry on R1,par-)
PMB119 PMB107 pPM41/pST29 (Plac-Citrus on mini-F; Plac-mCherry on R1,par-)
PMB120 PMB14 pPM42 (Ptet-mCherry on mini-F)
PMB121 MC1061 pPM43
PMB122 MC1061 pPM44
PMB123 MC1061 pPM45
PMB124 MC1061 pPM46
PMB125 PMB14 pPM43 (Ptet-YPet on pSC101, ts-Rep)
PMB126 PMB27 pPM44 (Plac-YPet on pSC101, ts-Rep)
PMB127 PMB14 pPM45 (Ptet-YPet on pSC101)
PMB128 PMB27 pPM46 (Plac-YPet on pSC101)
PMB129 PMB14 pST26/pPM30
PMB130 PMB27 pST26/pPM30 (Ptet-Citrus on R1, par-; Ptet-mCherry on pSC101)
PMB131 PMB14 pPM11
PMB132 PMB27 pPM12

Box3

Name Background Genotype Plasmid(s) Note
PMB133 PMB14 pST26/pPM42
PMB134 ST51 pST26 ST51 is CSH50/pKG339 (Plac-copA from Gerdes)
PMB135 ST51 pST28
PMB136 PMB14 pST26/pKG339
PMB137 PMB112 pKG339
PMB138 PMB114 pKG339
PMB139 ST52 pST26
PMB140 ST50 pKG339
PMB141 ST51 pKG491
PMB142 ST51 pKN1562
PMB143 ST51 pST27
PMB144 PMB12 pST52
PMB146 PMB27 pST52
PMB147 PMB27 pST52/pKN1562
PMB148 PMB27 pKG339
PMB149 PMB27 pKG339/pST26
PMB150 PMB27 pKG339/pKG491
CSH50 "CHS50" generated by curing plasmid from ST51
PMB151 CSH50 pST52
PMB152 CSH50 pST52/pKN1562
PMB153 CSH50 pKG339
PMB154 CSH50 pKG339/pKN1562
PMB155*** CSH50 pKG339/pKG491 ***(may be bad?!AmpR/IPTG-res subpopulation)
PMB156 PMB27 pST26
PMB157 PMB27 pKG491
PMB158 MC1061 pPM47
PMB159 MC1061 pPM48
PMB160 MC1061 attTn7::Ptet-mCherry
PMB161 MC1061 attTn7::Plac-mCherry
PMB162 PMB27 attTn7::Ptet-mCherry
PMB163 PMB27 attTn7::Plac-mCherry
PMB164 PMB14 attTn7::Ptet-mCherry
PMB165 PMB27 attTn7::Ptet-mCherry pST26
PMB166 PMB27 attTn7::Plac-mCherry pST27
PMB167 PMB14 attTn7::Ptet-mCherry pST26
PMB168 PMB27 attTn7::Ptet-mCherry pST26/pST52
PMB169 PMB27 attTn7::Plac-mCherry pST27/pST52
PMB170 PMB14 attTn7::Ptet-mCherry pST26/pST52
PMB171 MC1061 pKGCm
PMB173 CSH50 pKGCm
PMB175 MC4100 pKGCm
PMB177 AB1157 pKG339
PMB178 AB1157 pKG339/pKG491
PMB179 MC4100 pKG339
PMB180 MC4100 pKG339/pKG491
PMB181 MG1655 pKG339
PMB182 MG1655 pKG339/pKG491
PMB183 W3110 pKG339
PMB184 W3110 pKG339/pKG491
PMB185 MC1061 forthcoming pPM49
PMB186 MC1061 pPM50
PMB187 MC1061 pPM51
PMB188 MC1061 pPM52
PMB189 MC1061 pPM53
PMB190 MC1061 pPM54

Box4

Name Background Genotype Plasmid(s) Note
PMB191 CSH50 pPM54
PMB192 DJ480 pKG339
PMB193 DJ480 pPM54
PMB194 CSH50 pKG339/pPM50
PMB195 CSH50 pKG339/pPM51
PMB196 CSH50 pKG339/pPM52
PMB197 CSH50 pPM54/pKG491
PMB198 DJ480 pKG339/pKG491
PMB199 DJ480 pPM54/pPM50
PMB200 DJ480 pPM54/pPM51
PMB201 DJ480 pPM54/pPM52
PMB202 DJ480 pPM54/pKG491
PMB203 MC1061 pPM55
PMB204 MC1061 pPM56 clone 1 (also preserved PMB204 with clone 3)
PMB205 MC1061 pPM57
PMB206 MC1061 pPM58
PMB207 MC1061 pPM59
PMB208 PM27 pPM60
PMB209 PM27 pPM61
PMB210 PM27 pPM62
PMB211 PM27 pPM63
PMB212 PM27 pPM64
PMB213 PM27 pPM65
PMB214 PM27 pPM66
PMB215 PM27 pPM67
PMB216 MC1061 pPM68
PMB217 DJ480 pPM54/pPM59
PMB218 PMB27 pPM70
PMB219 PMB27 pPM71
PMB220 PMB27 pPM72
PMB221 PMB27 pPM73
PMB222 PMB27 pPM74
PMB223 CSH50 (Gerdes) ΔfimA::kan P1 trans. from Keio KO collection
PMB224 CSH50 (CGSC 8085) ΔfimA::kan P1 trans. from Keio KO collection
PMB225 MG1655seq (CGSC 7740) ΔfimE::kan P1 trans. from Keio KO collection
PMB226 MG1655seq (CGSC 7740) ΔthiC::kan P1 trans. from Keio KO collection
PMB227 PMB223 pKG491
PMB228 CGSC 8085 pKG491
PMB229 PMB224 pKG491
PMB230 PMB225 pKG491
PMB231 PMB226 pKG491
PMB232 CGSC 6412 pKG491
PMB233 PMB223 pKG491/pKG339
PMB234 CGSC 8085 pKG491/pKG339
PMB235 PMB224 pKG491/pKG339
PMB236 PMB225 pKG491/pKG339
PMB237 PMB226 pKG491/pKG339
PMB238 CGSC 6412 pKG491/pKG339
PMB239 CSH50 (KG) ΔfimA made by P1 from Keio, marker removed
PMB240 CSH50 (KG) ΔfimA pPM54 made by P1 from Keio, marker removed
PMB241 CSH50 (CGSC# 8085) ΔfimA made by P1 from Keio, marker removed
PMB242 CSH50 (CGSC# 8085) ΔfimA pPM54
PMB243 CSH50 (CGSC# 8085) pPM54
PMB244 MG1655(seq) ΔfimA made by P1 from Keio, marker removed
PMB245 MG1655(seq) ΔfimH made by P1 from Keio, marker removed
PMB246 CSH50 (KG) ΔfimH made by P1 from Keio, marker removed
PMB247 CSH50 (KG) DfimH pPM54
PMB248 ZK4 recA56 pPM54 MC4100 background
PMB249 ZK356 recA1 pPM54 MC1000 background
PMB250 MC1061 pPM75
PMB251 MC1061 pPM76
PMB252 MC1061 pPM77
PMB253 CGSC# 7740 pKG491
PMB254 DJ480 pKG491
PMB255 PMB239 pPM54/pKG491
PMB256 PMB246 pPM54/pKG491
PMB257 MC1061 pPM78 clone 1
PMB258 MC1061 pPM79 clone 3'
PMB259 MC1061 pPM80-3
PMB260 MC1061 pPM82 clone 1
PMB261 MC1061 pPM83 clone 1 (also preserved PMB261 with clone 2)
PMB262 MC1061 pPM84 clone 3
PMB263 MC1061 pPM85 clone 2
PMB264 MC1061 pPM86
PMB265 MC1061 pPM87
PMB266 MC1061 pPM80-2
PMB267 MC1061 pPM90 clone 4
PMB268 MC1061 pPM88 clone 2
PMB269 MC1061 pPM89 clone 1
PMB270 MC1061 pPM88 clone 1

Box5

Name Background Genotype Plasmid(s) Note
PMB271 CSH50(KG) pKG491/pPM82
PMB272 CSH50(KG) pKG491/pPM75
PMB273 CSH50(KG) pKG491/pPM83
PMB274 DJ480 pKG491/pPM82
PMB275 DJ480 pKG491/pPM75
PMB276 DJ480 pKG491/pPM83
PMB277 CGSC#7740 pKG491/pPM54
PMB278 CGSC#7740 pKG491/pPM82
PMB279 CGSC#7740 pKG491/pPM75
PMB280 CGSC#7740 pKG491/pPM83
PMB281 CSH50 pPM78
PMB282 DJ480 pPM78
PMB283 CSH50 pPM77
PMB284 DJ480 pPM77
PMB285 CSH50 pPM86
PMB286 DJ480 pPM86
PMB287 CSH50 pPM87
PMB288 DJ480 pPM87
PMB289 CSH50 pPM88-1
PMB290 DJ480 pPM88-1
PMB291 CSH50 pPM89
PMB292 DJ480 pPM89
PMB293 MC1061 pPM80-5
PMB294 MC1061 pPM80-6
PMB295 MC1061 pPM81 clone 4
PMB296 MC1061 pPM91
PMB297 MC1061 pPM92
PMB298 MC1061 pPM93
PMB299 MC1061 pPM94
PMB300 MC1061 pPM95
PMB301 MC1061 pPM96
PMB302 MC1061 pPM97
PMB303 MC1061 pPM98
PMB304 PMB239 pPM82
PMB305 PMB239 pPM75
PMB306 PMB239 pPM83
PMB307 PMB239 pKG491
PMB308 PMB246 pPM82
PMB309 PMB246 pKG491
PMB310 DJ480 pPM83
PMB311 DJ480 pKGCm
PMB312 PMB239 pPM54/pKG491 (made from PMB240)
PMB313 PMB239 pPM82/pKG491 (made from PMB304)
PMB314 PMB239 pPM82/pPM91 (made from PMB304)
PMB315 PMB239 pPM82/pPM93 (made from PMB304)
PMB316 PMB239 pPM82/pPM94 (made from PMB304)
PMB317 PMB239 pPM82/pPM97 (made from PMB304)
PMB318 PMB239 pPM82/pPM98 (made from PMB304)
PMB319 PMB239 pPM75/pKG491 (made from PMB305)
PMB320 PMB239 pPM83/pKG491 (made from PMB306)
PMB321 PMB239 pKG491/pPM83 (made from PMB307)
PMB322 PMB239 pKG491/pKGCm (made from PMB307)
PMB323 DJ480 pKGCm/pKG491 (made from PMB311)
PMB324 MC1061 pPM99
PMB325 MC1061 pPM100
PMB326 MC1061 pPM101
PMB327 MC1061 pPM102
PMB328 MC1061 pPM103A
PMB329 MC1061 pPM103B
PMB330 MC1061 pPM106A
PMB331 MC1061 pPM107A
PMB332 MC1061 pPM110A
PMB333 MC1061 pPM110B
PMB334 MC1061 pPM111A
PMB335 DJ480 pKG491/pPM106A made from PMB254
PMB336 DJ480 pKG491/pPM107A made from PMB254
PMB337 DJ480 pKG491/pPM110A made from PMB254
PMB338 DJ480 pKG491/pPM110B made from PMB254
PMB339 DJ480 pKG491/pPM111A made from PMB254
PMB340 CSH50 pKG491/pPM106A made from ST50
PMB341 CSH50 pKG491/pPM107A made from ST50
PMB342 CSH50 pKG491/pPM110A made from ST50
PMB343 CSH50 pKG491/pPM110B made from ST50
PMB344 CSH50 pKG491/pPM111A made from ST50
PMB345 CSH50 pKG491/pPM54/pKGCm made from PMB197
PMB346 DJ480 pKG491/pPM54/pKGCm made from PMB202
PMB347 PMB246 pKG491/pPM54/pKGCm made from PMB256

Box 6

Name Background Genotype Plasmid(s) Note
PMB348 MC1061 pPM93T-1
PMB349 MC1061 pPM93T-2
PMB350 MC1061 pPM95T-2
PMB351 MC1061 pPM95T-3
PMB352 MC1061 pPM99T-1
PMB353 MC1061 pPM99T-2
PMB354 MC1061 pPM101T-1
PMB355 MC1061 pPM101T-2
PMB357 DJ480 pPM54/pPM90 made from PMB193
PMB358 DJ480 pPM54/pPM95T-2 made from PMB193
PMB359 DJ480 pPM54/pPM95T-3 made from PMB193
PMB360 DJ480 pPM54/pPM101T-1 made from PMB193
PMB361 CSH50 DfimH pPM54/pPM81 made from PMB247
PMB362 CSH50 DfimH pPM54/pPM93-1 made from PMB247
PMB363 CSH50 DfimH pPM54/pPM93T-2 made from PMB247
PMB364 CSH50 DfimH pPM54/pPM94 made from PMB247
PMB365 CSH50 DfimH pPM54/pPM91-3 made from PMB247
PMB366 CSH50 DfimH pPM54/pPM99 made from PMB247
PMB367 CSH50 DfimH pPM54/pPM99T-1 made from PMB247
PMB368 CSH50 DfimH pPM54/pPM99T-2 made from PMB247
PMB369 CSH50 DfimH pPM54/pPM100 made from PMB247
PMB370 CSH50 DfimH pKG491/pPM106A made from PMB309
PMB371 CSH50 DfimH pKG491/pPM107A made from PMB309
PMB372 MC1061 pPM112
PMB373 MC1061 pPM113
PMB374 DJ480 pPM82
PMB375 DJ480 pPM106A
PMB376 DJ480 pPM107A
PMB377 MC4100 pPM54
PMB378 MC4100 pPM82
PMB379 MC4100 pPM106A
PMB380 MC1061 pPM114 clone 1
PMB381 MC1061 pPM115 clone 1
PMB382 MC1061 pPM95T-7
PMB383 MC1061 pPM95T-8
PMB384 MC1061 pPM116 clone 1
PMB385 MC1061 pPM117 clone 1
PMB386 MC1061 pPM118 clone 1
PMB387 MC1061 pPM119 clone 1
PMB388 MC1061 pPM120 clone 1
PMB389 MC1061 pPM121* clone 1
PMB390 MC1061 pPM122 clone 2
PMB391 MC1061 pPM123 clone 3
PMB392 MC1061 pPM124 clone 1
PMB393 MC1061 pPM125 clone 1
PMB394 MC1061 pPM126 clone 2
PMB395 MC1061 pPM127 clone 3
PMB396 MC1061 pPM130 clone 1
PMB397 MC1061 pPM131 clone 1
PMB398 MC1061 pPM132 clone 1
PMB399 MC1061 pPM133 clone 1
PMB400 MC1061 pPM134 clone 1
PMB401 DJ480 pPM54/pPM81 made from PMB193
PMB402 DJ480 pPM54/pPM90 made from PMB193
PMB403 DJ480 pPM54/pPM112 made from PMB193
PMB404 DJ480 pPM54/pPM113 made from PMB193
PMB405 DJ480 pPM54/pPM122 made from PMB193
PMB406 DJ480 pPM54/pPM123 made from PMB193
PMB407 DJ480 pPM54/pPM124 made from PMB193
PMB408 DJ480 pPM54/pPM125 made from PMB193
PMB409 DJ480 pPM54/pPM126 made from PMB193
PMB410 DJ480 pPM54/pPM127 made from PMB193
PMB411 DJ480 pPM54/pPM131 made from PMB193
PMB412 DJ480 pPM54/pPM132 made from PMB193
PMB413 DJ480 pPM54/pPM133 made from PMB193
PMB414 DJ480 pPM54/pPM134 made from PMB193
PMB415 MC1061 pPM135 clone 1
PMB416 MC1061 pPM136 clone 2
PMB417 MC1061 pPM137 clone 1
PMB418 MC1061 pPM138 clone 1
PMB419 MC1061 pPM139 clone 1
PMB420 MC1061 pPM140 clone 9
PMB421 MC1061 pPM141 clone 2
PMB422 MC1061 pKG491
PMB423 MC1061 pKN1562
PMB424 DJ480 pPM54/pACT7 made from PMB193


Box 7

Name Background Genotype Plasmid(s) Note
PMB425 DJ480 pPM54/pPM135 made from PMB193
PMB426 DJ480 pPM54/pPM136 made from PMB193
PMB427 DJ480 pPM54/pPM137 made from PMB193
PMB428 DJ480 pPM54/pPM138 made from PMB193
PMB429 DJ480 pPM54/pPM139 made from PMB193
PMB430 DJ480 pPM54/pPM140 made from PMB193
PMB431 DJ480 pPM54/pPM141 made from PMB193
PMB432 MC1061 pPM91-3
PMB433 DJ480 pPM106A/pPM90 made from PMB375
PMB434 DJ480 pPM106A/pPM113 made from PMB375
PMB435 DJ480 pPM106A/pPM135 made from PMB375
PMB436 DJ480 pPM106A/pPM136 made from PMB375
PMB437 DJ480 pPM106A/pPM137 made from PMB375
PMB438 DJ480 pPM106A/pPM138 made from PMB375
PMB439 DJ480 pPM106A/pPM139 made from PMB375
PMB440 DJ480 pPM106A/pPM140 made from PMB375
PMB441 DJ480 pPM106A/pPM141 made from PMB375
PMB442 DJ480 pPM106A/pPM122 made from PMB375
PMB443 DJ480 pPM106A/pPM123 made from PMB375
PMB444 DJ480 pPM106A/pACT7 made from PMB375
PMB445 DJ480 pPM54/pACT7/pPM135 made from PMB424
PMB446 DJ480 pPM54/pACT7/pPM136 made from PMB424
PMB447 DJ480 pPM54/pACT7/pPM140 made from PMB424
PMB448 DJ480 attTn7::PLtet-mCherry made using pPM47 for integration
PMB449 MG1655 attTn7::PLtet-mCherry made using pPM47 for integration
PMB450 MC4100 attTn7::PLtet-mCherry made using pPM47 for integration
PMB451 DJ480 pPM106A/pPM137 made from PMB375; remake of PMB437
PMB452 DJ480 pPM107A/pPM137 made from PMB376
PMB453 DJ480 attTn7::PLtet-mCherry pPM54 made from PMB448
PMB454 DJ480 attTn7::PLtet-mCherry pPM106A made from PMB448
PMB455 DJ480 pPM54/pPM142 (clone 7) made from PMB193
PMB456 DJ480 pUA66
PMB457 DJ480 pUA66/pPM136
PMB458 DJ480 pUA66/pPM137
PMB459 DJ480 pUA66/pPM139
PMB460 DJ480 pPM136
PMB461 DJ480 pPM137
PMB462 DJ480 pT7POL23
PMB463 DJ480 pPM136/pT7POL23 made from PMB460
PMB464 DJ480 pPM136/pT7POL26 made from PMB460
PMB465 MC4100 pPM54/pPM136 made from PMB377
PMB466 MC4100 pPM54/pPM137 made from PMB377
PMB467 MC4100 pPM54/pPM139 made from PMB377
PMB468 MC4100 pPM54/pPM140 made from PMB377
PMB469 MC4100 pPM54/pPM141 made from PMB377
PMB470 MC4100 pPM54/pPM142 made from PMB377
PMB471 DJ480 araB::T7RNAP_tetA pPM54/pPM136 made from PMB479
PMB472 MC4100 araB::T7RNAP_tetA pPM54/pPM136 made from PMB480
PMB473 DJ480 araB::T7RNAP_tetA P1 from BL21-AI to DJ480
PMB474 MC4100 araB::T7RNAP_tetA P1 from BL21-AI to MC4100
PMB475 DJ480 araB::T7RNAP_tetA pPM54/pPM140 made from PMB479
PMB476 MC4100 araB::T7RNAP_tetA pPM54/pPM140 made from PMB480
PMB477 DJ480 araB::T7RNAP_tetA pPM136 made from PMB473
PMB478 MC4100 araB::T7RNAP_tetA pPM136 made from PBM474
PMB479 DJ480 araB::T7RNAP_tetA pPM54 made from PMB473
PMB480 MC4100 araB::T7RNAP_tetA pPM54 made from PBM474
PMB481 MC4100 araB::T7RNAP_tetA pPM54/pPM136/pLysS made from PBM472
PMB482 DJ480 attP::tetR & lacIq DJ480 transduced with P1 from PMB14
PMB483 DJ480 ΔacrA, attP::tetR & lacIq ST88 transduced with P1 from PMB14
PMB484 DJ480 attP::tetR & lacIq pPM54 trf of PMB482
PMB485 DJ480 ΔacrA, attP::tetR & lacIq pPM54 trf of PMB483
PMB486 DJ480 attP::tetR & lacIq pPM54/pPM93 trf of PMB484
PMB487 DJ480 attP::tetR & lacIq pPM54/pPM95 trf of PMB484
PMB488 DJ480 ΔacrA, attP::tetR & lacIq pPM54/pPM93 trf of PMB485
PMB489 DJ480 ΔacrA, attP::tetR & lacIq pPM54/pPM95 trf of PMB485
PMB490 TOP10 pCP20 from Dirk (Datsenko & Wanner)
PMB491 BW27783 araB::T7RNAP_tetA P1 from PMB14 into BW27783 from Raul (nalR?)
PMB492 BW27783 araB::T7RNAP_tetA pPM54 made from PMB491
PMB493 BW27783 araB::T7RNAP_tetA pPM54/pPM136 made from PMB492
PMB494 BW27783 araB::T7RNAP_tetA pPM54/pPM140 made from PMB492

Box 8

Name Background Genotype Plasmid(s) Note
PMB495 MC1061 pPM144 clone 3
PMB496 MC1061 pPM145 clone 1
PMB497 MC1061 pPM146 clone 1
PMB498 MC1061 pPM147 clone 2
PMB499 MC1061 pPM148 clone 1
PMB500 MC1061 pPM149 clone 1
PMB501 MC1061 pPM150 clone 1
PMB502 MC1061 pPM151 clone 1
PMB503 MC1061 pPM152 clone 1
PMB504 MC1061 pPM153 clone 1
PMB505 MC1061 pPM154 clone 3
PMB506 MC1061 pPM155 clone 1
PMB507 MC1061 pPM156 clone 1
PMB508 MC1061 pPM157 forthcoming
PMB509 MC1061 pPM158 clone 1
PMB510 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM144
PMB511 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM148
PMB512 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM152
PMB513 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM153
PMB514 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM156
PMB515 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM158
PMB516 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM144
PMB517 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM148
PMB518 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM152
PMB519 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM153
PMB520 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM156
PMB521 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM158
PMB522 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM156/pM152 made from PMB514; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml)
PMB523 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM156/pM152 made from PMB520; selected on LBgluc(0.2%) / carbenicillin(20ug/ml) / Cm(3ug/ml) / Kn(35ug/ml)
PMB524 MC1061 pPM160 clone 1
PMB525 MC1061 pPM161 clone 1
PMB526 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM160
PMB527 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM161
PMB528 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM160
PMB529 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM161
PMB530 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM160/pPM153
PMB531 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM161/pPM152 pSC101_Plac-copA_Kn / R1(-)_Pt7-gfpmut3_Cm / R1(-)_Pt7-mCherry_Ap
PMB532 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM160/pPM153
PMB533 PMB492 BW27783 araB::T7RNAP_tetA pPM54/pPM161/pPM152
PMB535 MC1061 pPM145 A5 clone A+5; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25
PMB536 MC1061 pPM145 A6 clone A+6; from original transformation, restruck on Ap20; glycerol stock cultured in LB/Ap25
PMB537 MC1061 pPM162 clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25
PMB538 MC1061 pPM163 clone-1; selected on Ap10, restruck on Ap20; glycerol stock cultured in LB/Ap25
PMB539 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM160-1 redo of PMB526. Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn)
PMB540 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM160-2 Selected on Kn35/Cm8, restruck on same; glycerol stock cultured in LB/Cm5 (forgot Kn)
PMB541 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM145 (A5) selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
PMB542 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM162-1 selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
PMB543 PMB479 DJ480 araB::T7RNAP_tetA pPM163-1(1) clone-1, selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
PMB544 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM163-1(2) clone-2(small), selected on Ap20, restruck on Ap20 with Kn5 overlay; glycerol stock cultured in LBglucose/Kn35/Cb25
PMB545 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM145 A5-2 selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
PMB546 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM163-1a selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20; not the same as PMB543=479/163-1(1)
PMB547 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM163-1b selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
PMB548 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM163-1c selected on Ap20, stored in fridge, restruck on Ap20; glycerol stock cultured in LBglucose/Kn35/Cb20
PMB549 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM145 (A5) 145A5 plasmid prep from PMB535 grown in Cb20; trfs selected on Ap10; glycerol stock cultured in LBglucose/Cb10 (forgot Kn)
PMB550 MC1061 pPM164 (2) - no good clone 2; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
PMB551 MC1061 pPM164 (3) - no good clone 3; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
PMB552 MC1061 pPM165 (4) clone 4; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
PMB553 MC1061 pPM165 (5) - no good clone 5; trfs selected on Ap50; restruck on Ap12.5; glycerol stock cultured in LB/Ap50
PMB554 MC1061 pPM166 clone 25-6; trfs selected on LB/Ap25; glycerol stock cultured in LB/Cb10
PMB555 MC1061 pPM167 clone 80-6; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10
PMB556 MC1061 pPM168 clone 80-7; trfs selected on LB/Ap80; glycerol stock cultured in LB/Cb10
PMB557 MC1061 pPM169 clone T1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB558 MC1061 pPM170 clone T2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB559 MC1061 pPM171 clone T3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB560 MC1061 pPM172 clone E1-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB561 MC1061 pPM173 clone E2-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB562 MC1061 pPM174 clone E3-1; trfs selected on LB/Ap12; restruck on LBglucose/Ap20; glycerol stock cultured in LB/Ap20
PMB563 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM165 trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
PMB564 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM169 trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
PMB565 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM170 trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
PMB566 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM171 trfs selected and restruck on LBglucose/Kn35/Ap10; glycerol stock cultured in LBglucose/Ap20
PMB567 MC1061 pPM175 trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
PMB568 MC1061 pPM176 trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
PMB569 MC1061 pPM177 trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
PMB570 MC1061 pPM178 trfs selected on LB/Ap12; glycerol stock cultured in LB/Ap25
PMB571 MC1061 pPM179 trfs selected on LB/Ap25; restruck on LB/Ap20; glycerol stock cultured in LB/Ap25
PMB572 PMB479 DJ480 araB::T7RNAP_tetA pPM54/pPM179 selected on LBgluc(0.2%)/Kn35/Ap20, grown at 30°C; glycerol stock cultured in LB/Cb25

Box 9

Name Background Genotype Plasmid(s) Note
PMB573 MG1655 DJ480 araB::T7RNAP_tetA pKGCm made from PMB473; selected on LBgluc(0.2%)/Cm15
PMB574 MG1655 DJ480 araB::T7RNAP_tetA pPM136/pKGCm made from PMB477; selected on LBgluc(0.2%)/Ap40/Cm15
PMB575 MG1655 DJ480 araB::T7RNAP_tetA pPM54/pKGCm made from PMB479; selected on LBgluc(0.2%)/Kn35/Cm8
PMB576 MG1655 DJ480 araB::T7RNAP_tetA pPM54/pPM136/pKGCm made from PMB471; selected on LBgluc(0.2%)/Kn35/Cb32/Cm4
PMB577 MG1655 DJ480 araB::T7RNAP_tetA pPM54/pPM170/pKGCm made from PMB565; selected on LBgluc(0.2%)/Kn35/Cb32/Cm4
PMB578 MC1061 pPM180 clone 1
PMB579 MC1061 pPM181 clone 1
PMB580 MC1061 pAM238 SpcR
PMB581 MC1061 pNG162 SpcR
PMB582 MC1061 pNG162a SpcR
PMB583 MC1061 pKG339 TetR
PMB584 MC1061 pPM182 SpcR; clone 1-1
PMB585 MC1061 pPM183 SpcR; clone 2-1
PMB586 MC1061 pPM184 SpcR; clone 5-1
PMB587 MC1061 pPM185 SpcR; clone 6-1
PMB588 PMB473 pPM182 SpcR; clone 1-1
PMB589 PMB473 pPM183 SpcR; clone 2-1
PMB590 PMB473 pKGCm/pPM184 SpcR; clone 5-1
PMB591 PMB473 pKGCm/pPM185 SpcR; clone 6-1
PMB592 MC1061 pPM186 SpcR; clone A-1
PMB593 MC1061 pPM187 SpcR; clone B-1
PMB594 PMB473 pPM186
PMB595 PMB473 pPM187
PMB596 PMB473 pPM136/pPM182 made from PMB477
PMB597 PMB473 pPM136/pPM183 made from PMB477
PMB598 PMB473 pPM136/pPM186 made from PMB477
PMB599 PMB473 pPM136/pPM187 made from PMB477
PMB600 PMB473 pKGCm/pPM186 made from PMB573
PMB601 PMB473 pKGCm/pPM187 made from PMB573
PMB602 PMB473 pKGCm/pPM136/pPM184 made from PMB574
PMB603 PMB473 pKGCm/pPM136/pPM185 made from PMB574
PMB604 PMB473 pKGCm/pPM136/pPM186 made from PMB574
PMB605 PMB473 pKGCm/pPM136/pPM187 made from PMB574

Nate's Strains

Name Background Genotype Plasmid(s) Note Notebook Page
NDL-1 One Shot 2T1R (Invitrogen) ccdB survival cells. Recovered from pGRG37 by curing. L.2.14
NDL-2 MC4100 Lac- pNDL-17, Alon 2-A-6 e.9.2
NDL-3 MC4100 Lac- pNDL-17, Alon 11-A-6 E.9.2
NDL-4 MC4100 Lac- pNDL-17, Alon 13-B-9 E.9.2
NDL-5 MC4100 Lac- pNDL-17, Alon 18-E-2 E.9.2
NDL-6 MG1655 pNDL-18 Promoterless GFP, +CFP, -term L.6.14
NDL-7 MG1655 pNDL-19 Promoterless GFP,-CFP, +term L.6.14
NDL-8 MG1655 pNDL-20 promoterless GFP,+term,+CFP L.6.14
NDL-9 MG1655 attTn7::(no promoter)-GFPmut2 Generated by curing NDL-6 of pNDL-18 L.6.28
NDL-10 MG1655 attTN7::B0015-(no promoter)-GFPmut2 Generated by curing NDL-7 of pNDL-19 L.6.28
NDL-11 MG1655 attTn7::B0015-(no promoter)-GFPmut2 Generated by curing NDL-8 of pNDL-20 L.6.28
NDL-12 MG1655 pNDL-21 Bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-13 MG1655 pNDL-22 Bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-14 MG1655 pNDL-23 very bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-15 MG1655 pNDL-24 very bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-16 MG1655 pNDL-25 Intermediate bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-17 MG1655 pNDL-26 Intermediate bright ColE1 RNA1-tdCherry on R1 L.7.19
NDL-18 MG1655 pNDL-31 Bright ColE1 RNA1-tdCherry in pNDL6 integration vector L.11.11
NDL-19 MG1655 pNDL-32 Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vecotr L.11.11
NDL-20 MG1655 pNDL-33 Very bright ColE1 RNA1-tdCherry in pNDL-6 integration vector L.11.11
NDL-21 MG1655 attTn7::pRNA1(bright)-tdCherry none bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-31) L.11.19
NDL-22 MG1655 attTn7::pRNA1(very bright)-tdCherry none Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-32) L.11.19
NDL-23 MG1655 attTn7::pRNA1(very bright)-tdCherry none Very bright ColE1 RNA1-tdCherry integrated at Tn7 (cured of pNDL-33) L.11.19
NDL-24 MG1655 pNDL-41 Contains plasmid with FRT-flanked KanR gene. For Integrations.
NDL-25 DH5-Alpha 'WT' none Stock for cloning strain-- plain DH5 alpha cells.
NDL-26 BL21-AI (Invitrogen) pNDL-44 Contains plasmid with T7 His-bt-B-gal expression construct 39.25
NDL-27 MG1655 pNDL-46 Strain for integration of 1-E-3 Alon Clone L.17.1
NLD-28 MG1655 pNDL-47 Strain for Integration of 1-E-5 Alon Clone L.17.1
NDL-29 MG1655 pNDL-48 Strain for Integration of 2-A-6 Alon Clone L.17.1
NDL-30 MG1655 pNDL-49 Strain for Integration of 2-C-11 Alon Clone L.17.1
NDL-31 MG1655 pNDL-50 Strain for Integration of 2-F-4 Alon Clone L.17.1
NDL-32 MG1655 none NDL-27 cured of pNDL-46. Alon clone integrant. L.17.2
NDL-33 MG1655 none NDL-28 cured of pNDL-47. Alon clone integrant. L.17.2
NDL-34 MG1655 none NDL-29 cured of pNDL-48. Alon clone integrant. L.17.2
NDL-35 MG1655 none NDL-30 cured of pNDL-49. Alon clone integrant. L.17.2
NDL-36 MG1655 none NDL-31 cured of pNDL-50. Alon clone integrant. L.17.2
NDL-37 BL-21 lacY1 mutation (Novagen says its a LacZY mutation) none LacZ- strain for protein expression. Used in purificaton of btB-gal 42.1
NDL-38 NDL-37 (Novagen Tuner DE3) lacY1 mutation (Novagen says its a LacZY mutation) pNDL-44 For purification of btB-gal 42.1
NLD-39 MG1655 Wanner strain for integration into chromosome pKD46 (pNDL-???)
NDL-40 MG1655 pNDL-52 pRNA1-mCherry integrated downstream of attTn7 cured of pKD46 Constitutive mCherry strain for library, prior to P1 transduction L.18.6
NDL-41 BL-21 AI Contains Ara-Inducible T7 Polymerase pNDL-96 For expression of GST-cotA 43.9
NDL-42 Rosetta2 DE3 (Novagen) BL21 derived Strain from Novagen with codon+ plasmid pRARE2 For recombinant protein expression with rare codons L.47.1
NDL-43 MG1655 (CGSC 7740) Fully sequenced MG1655 Strain none Host strain for library construction L.18.15
NDL-44 Tuner BL21 (NDL-37) BL21 (DE3) lacZY mutant pRARE2 (camR) Codon+ Tuner Strain ex.48.2
NDL-45 MG1655 near attTn7::pBR322 pRNA1-mCherry-FRT-KanR None Product of P1 from NDL-40 into MG1655 (NDL-43) L.18.15
NDL-46 Tuner BL21 (NDL-37) BL21 (DE3) LacZY mutant pRARE2 (camR), pNDL-44 (ampR) Strain for expression of His-bt-B-gal 48.3
NDL-47 MG1655 near attTn7::pRNA1-tdCherry none FLP-removed KanR from NDL-45 L.18.9
NDL-48 BW25113 ΔMotB::KanR none Keio ΔMotB strain CGSC 9564 L.19.1
NDL-49 BW25113 ΔMotA::KanR none Keio ΔMotA strain CGSC 9564 L.19.1
NDL-50 BW25113 ΔFliC::KanR none Keio ΔfliC strain CGSC 9586 L.19.1
NDL-51 NDL-47(-3) ΔMotB::kanR near attTn7::pRNA1-mCherry none NDL-47 with keio 9564 deletion L.19.5
NDL-52 NDL-47(-3) ΔMotA::kanR near attTn7::pRNA1-mCherry none NDL-47 with keio 9565 deletion L.19.5
NDL-53 NDL-47(-3) ΔfliC::kanR near attTN7::pRNA1-mCherry none NDL-47 with keio 9586 deletion L.19.5
NDL-54 MG1655 ΔMotA::kanR none (pKD46 cured) MG1655 with keio 9565 deletion L.20.5
NDL-55 MG1655 ΔMotB::kanR none (pKD46 cured) MG1655 with keio 9564 deletion L.20.5
NDL-56 NDL-47 ΔMotA::kanR near attTn7::pRNA1-mCherry none P1vir:NDL-54 -> NDL-47 L.20.7
NDL-57 NDL-47 ΔmotB::kanR near attTn7::pRNA1-mCherry none p1vir:NDL-55 -> NDL-47 L.20.7
NDL-58 NDL-47 ΔmotA near attTn7::pRNA1-mCherry none NDL-56 following FLP removal of KanR L.20.11
NDL-59 NDL-47 ΔmotB near attTn7::pRNA1-mCherry none NDL-57 following FLP removal of KanR L.20.11
NDL-93 MG1655 ΔmotA none NDL-54 following FLP removal of KanR L.24.6
NDL-94 MG1655 ΔmotB none NDL-55 following FLP removal of KanR L.24.6
NDL-95 BW25113 ΔlacI::KanR none KanR lacI Keio strain T.2.1
NDL-96 MG1655 ΔlacI::KanR none P1vir-NDL-95 ---> MG1655 T.2.1
NDL-97 MG1655 DE3 (specR) none P1vir-PMB14----> MG1655 T.2.1
NDL-98 BL21 DE3 pNDL-99 (pGK8-GFP003} T.3.1
NDL-99 BL21 DE3 pNDL-100 (pGK8-GFP008} T.3.1
NDL-100 BL21 DE3 pNDL-101 (pGK8-GFP012} T.3.1
NDL-101 BL21 DE3 pNDL-102 (pGK8-GFP021} T.3.1
NDL-102 BL21 DE3 pNDL-103 (pGK8-GFP037} T.3.1
NDL-103 BL21 DE3 pNDL-104 (pGK8-GFP038} T.3.1
NDL-104 BL21 DE3 pNDL-105 (pGK8-GFP053} T.3.1
NDL-105 BL21 DE3 pNDL-106 (pGK8-GFP100} T.3.1
NDL-106 BL21 DE3 pNDL-107 (pGK8-GFP170} T.3.1
NDL-107 BL21 DE3 pNDL-108 (pGK8-GFP182} T.3.1
NDL-108 TB10 Wanner Strain with Heat Inducible Machinery none L.something
NDL-109 CP78 (CGSC 4695) thr,leu,his,arg auxotroph (relA WT) none T.5.1
NDL-110 CP79 (CGSC 4696) thr,leu,his,arg auxotroph (relA2-- null) none T.5.1
NDL-111 BW25113 (CGSC 10159) Keio RelA knockout (kanR) none T.5.1
NDL-153 CP78 (NDL-109,CGSC 4695) thr,leu,his,arg auxotroph (RelA WT) pKD46 Strain for Wanner Integration of GFP Reporters T.2.2
NDL-154 CP79 (NDL-110,CGSC 4696) thr,leu,his,arg auxotroph (relA2--null) pKD46 Strain for Wanner Integration of GFP Reporters T.2.2
NDL-155 TB10 heat inducible labmda red prophage none pRNA-1/mCherry-FRT-kanR integrant L.25.23
NDL-156 TB10 heat inducible labmda red prophage none pRNA-1/mCherry/mKate2 hybrid-FRT-kanR integrant L.25.23
NDL-157 TB10 heat inducible labmda red prophage none pRNA-1/mKate2-FRT-kanR integrant L.25.23
NDL-158 NDL-93 Δ motA none P1vir_NDL-155--> NDL-93 L.25.23
NDL-159 NDL-93 Δ motA none P1vir_NDL-156--> NDL-93 L.25.23
NDL-160 NDL-93 Δ motA none P1vir_NDL-157--> NDL-93 L.25.23
NDL-161 NDL-93 Δ motA near attTN7 pRNA1-mCherry none FLP removed kanR from NDL-158 L.25.31
NDL-162 NDL-93 Δ motA near attTN7 pRNA1-mCherry-mKate2 hybrid none FLP removed kanR from NDL-159 L.25.31
NDL-162 NDL-93 Δ motA near attTN7 pRNA1-mKate2 none FLP removed kanR from NDL-160 L.25.31
NDL-192 TB10 Xie lon-Venus fusion none From Sunney Xie Library
NDL-193 TB10 Xie AtpD-Venus fusion none From Sunney Xie Library
NDL-194 TB10 Xie lig-Venus fusion none From Sunney Xie Library
NDL-195 TB10 Xie GapA-Venus fusion none From Sunney Xie Library
NDL-196 B. Subtilis TMN-690 (pYqxm-mCherry, pHag-GFP) none from Tom Norman
NDL-197 B. Subtilis TMN-694 (pYqxm-mCherry, pHag-GFP, ΔslrR) none from Tom Norman
NDL-198 B. Subtilis TMN- none from Tom Norman
NDL-199 MG1655 (CGSC 7740) WT none Obtained from CGSC L.27.1
NDL-200 MG1655 (CGSC 6300) WT none Obtained from CGSC (3 colonies saved) L.27.1
NDL-201 MG1655 (CGSC 6300) near attTn7::pRNA1-mCherry-mKate2-kanR Hybrid none Obtained from transduction of NDL-200 with p1Vir.NDL-156 L.27.2
NDL-202 MG1655 (CGSC 6300) near attTn7::pRNA1-mCherry-mKate2 hybrid none obtained from NDL-201 by FLP-removal of kanR marker L.27.4
NDL-203 MG1655 (CGSC 6300) near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA-kanR none p1Vir.NDL-54--> NDL-202 L.27.7
NDL-204 MG1655 (CGSC 6300) near attTn7::pRNA1-mCherry-mKate2 hybrid. NDL-54 ΔmotA none obtained by FLP removal of kanR marker from NDL-203. NOTE: may still have pCP20. See L.27.8 L.27.8
NDL-205 NDL-162 near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library lon-Venus. See L.29.4 for ID. none P1vir.NDL-192--> NDL-162 L.29.2
NDL-206 NDL-162 near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library AtpD-Venus. See L.29.4 for ID. none P1vir.NDL-193--> NDL-162 L.29.2
NDL-207 NDL-162 near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library LigA-VenusSee L.29.4 for ID. none P1vir.NDL-194--> NDL-162 L.29.2
NDL-208 NDL-162 near attTn7::pRNA1-mCherry-mKate2, ΔmotA, Xie library gapA-Venus. See L.29.4 for ID. none P1vir.NDL-195--> NDL-162 L.29.2
NDL-209 Xie Library strain markerless ΔmotA integrated into NDL-192. none Wanner integrant. L.30.4
NDL-210 Xie Library strain markerless ΔmotA integrated into NDL-193. none Wanner integrant. L.30.4
NDL-211 Xie Library strain markerless ΔmotA integrated into NDL-194. none Wanner integrant. L.30.4
NDL-212 Xie Library strain markerless ΔmotA integrated into NDL-195. none Wanner integrant. L.30.4
NDL-237 BW25113 CGSC 8590, clpP Keio Knockout none From CGSC. L.34.1
NDL-238 BW25113 CGSC 8917, ihfB Keio Knockout none From CGSC. L.34.1
NDL-239 BW25113 CGSC 9111, hns Keio Knockout none From CGSC. L.34.1
NDL-240 BW25113 CGSC 9441, ihfA Keio Knockout none From CGSC. L.34.1
NDL-241 BW25113 CGSC 10954, groL Keio Knockout none From CGSC. L.34.1
NDL-242 NDL-162 MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid none NDL-162 transduced with P1Vir-NDL-237 L.34.2
NDL-243 NDL-162 MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid none NDL-162 transduced with P1Vir-NDL-238 L.34.2
NDL-244 NDL-162 MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid none NDL-162 transduced with P1Vir-NDL-239 L.34.2
NDL-245 NDL-162 MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid none NDL-162 transduced with P1Vir-NDL-240 L.34.2
NDL-246 NDL-162 MG1655 (CGSC7740) near attTn7::pRNA1-mCherry-mkate2 hybrid none NDL-162 transduced with P1Vir-NDL-241 L.34.2
NDL-247 NDL-192 Xie lon reporter (Xie Background), ΔmotA::kanR none P1vir.NDL-209 --> NDL192 L.30.7
NDL-248 NDL-193 Xie atpD reporter (Xie Background), ΔmotA::kanR none P1vir.NDL-210 --> NDL193 L.30.7
NDL-249 NDL-194 Xie lig reporter (Xie Background), ΔmotA::kanR none P1vir.NDL-211 --> NDL194 L.30.7
NDL-250 NDL-195 Xie gapA reporter (Xie Background), ΔmotA::kanR none P1vir.NDL-212 --> NDL195 L.30.7
NDL-254 TMN1090 amyE::Pyqxm-CFP (spcR) none TMN1090 transduced with amyE::Pyqxm-CFP (spcR)
NDL-255 TMN985 Δslr::mls none TMN985 transduced with Δslr::mls
NDL-256 TMN1049 TMN1049 Δslr::mls none TMN1049 transduced with Δslr::mls
NDL-257 NDL-254 (TMN1090 amyE::Pyqxm-CFP (spcR)) NDL-254 Δslr::mls none NDL-254 transduced with Δslr::mls
NDL-258 NDL-256 (TMN1049 Δslr::mls ) NDL-256 hagA233V none NDL-256 transduced with hagA233V (phleo)
NDL-259 NDL-192 near attTn7::mCherry-mkate2 hybrid specR none Wanner integration of constitutive red of NDL-162 L.31.20
NDL-260 NDL-194 near attTn7::mCherry-mkate2 hybrid specR none Wanner integration of constitutive red of NDL-162 L.31.20
NDL-261 NDL-195 near attTn7::mCherry-mkate2 hybrid specR none Wanner integration of constitutive red of NDL-162 L.31.20
NDL-262 TMN1095 TMN690 + pSpank-sinI (note: sinI - stop codon) none Renamed TMN1095.
NDL-263 TMN1096 TMN694 + pSpank-sinI (note: sinI - stop codon) none Renamed TMN1096.
NDL-264 TMN1101 TMN694 + > 2x sinI none Renamed TMN1101.
NDL-265 TMN1111 TMN694 + 2x sinI none Renamed TMN1111.
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