Penn State University 2006:Eikenplates: Difference between revisions
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='''Eiken Agar Plates'''= | ='''Eiken Agar Plates'''= | ||
Eiken plates should be made up the same day or no more than one day before using them. Otherwise they dry out and cells do not swarm. | |||
Procedure | |||
Add the following ingredients: | |||
*0.25g tryptone | *0.25g tryptone | ||
*0.20g NaCl | *0.20g NaCl | ||
| Line 9: | Line 15: | ||
Autoclave 20 min slow exhaust. | Autoclave 20 min slow exhaust. | ||
Add appropriate antibiotic | |||
This gives a final [eiken] of 0.20% | |||
[http://openwetware.org/wiki/IGEM:PennState Main] | [http://openwetware.org/wiki/IGEM:PennState Main] | ||
Revision as of 16:27, 26 October 2006
Eiken Agar Plates
Eiken plates should be made up the same day or no more than one day before using them. Otherwise they dry out and cells do not swarm.
Procedure
Add the following ingredients:
- 0.25g tryptone
- 0.20g NaCl
- 0.05 eiken agar
- 0.15g sodium citrate
- 25ml water
Autoclave 20 min slow exhaust.
Add appropriate antibiotic
This gives a final [eiken] of 0.20%
