Penn State University 2006:LBplates: Difference between revisions
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='''LB Plates'''= | |||
===<font color="red">LB Plates</font>=== | ===<font color="red">LB Plates</font>=== | ||
(per/L H2O) | (per/L H2O) | ||
Revision as of 21:47, 26 October 2006
LB Plates
LB Plates
(per/L H2O)
1. Weigh and add to H2O in large Erlenmeyer:
- 10 g Bacto-tryptone
- 5 g Bacto-yeast extract
- 10 g NaCl
- 15 g Bacto-agar
2. Autoclave on slow exhaust cycle 25 min
3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. (in sterile hood)
4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic.
5. Pour plates (~25 mL/plate)
6. Allow to solidify & store in bag in cold-room
