Penn State University 2006:Microchannel: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 22: | Line 22: | ||
*2. Run the coating procedure for 1 minute | *2. Run the coating procedure for 1 minute | ||
*3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)'' | *3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)'' | ||
[http://openwetware.org/wiki/IGEM:PennState Main] | |||
Revision as of 15:32, 26 October 2006
Polydimethylsiloxane (PDMS) Prep
- 1. Add 10g PDMS and 1g curing agent to a weigh boat
- 2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles)
- 3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone
Spin Coating
- 1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat
- 2. Attach the selected mold to the spin coating machine
- 3. Pour PDMS slowly and evenly over the entire mold
- 4. Spin the mold for 30 seconds at 500rpm
- 5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C
- 6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage)
PDMS Surface Treatment via O2 Plasma
- 1. Place the PDMS channels "face up" on the O2 Plasma device platform
- 2. Run the coating procedure for 1 minute
- 3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)
