Penn State University 2006:Microchannel: Difference between revisions
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''== Polydimethylsiloxane PDMS Prep == | '' | ||
== Polydimethylsiloxane PDMS Prep == | |||
1. Add 10g PDMS and 1g curing agent to a weigh boat | *1. Add 10g PDMS and 1g curing agent to a weigh boat | ||
2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles) | *2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles) | ||
3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone | *3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone | ||
== Spin Coating == | == Spin Coating == | ||
1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat | *1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat | ||
2. Attach the selected mold to the spin coating machine | *2. Attach the selected mold to the spin coating machine | ||
3. Pour PDMS slowly and evenly over the entire mold | *3. Pour PDMS slowly and evenly over the entire mold | ||
4. Spin the mold for 30 seconds at 500rpm | *4. Spin the mold for 30 seconds at 500rpm | ||
5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C | *5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C | ||
6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage) | *6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage) | ||
== PDMS | == PDMS Surface Treatment via O2 Plasma == | ||
1. Place the PDMS channels "face up" on the O2 Plasma device platform | *1. Place the PDMS channels "face up" on the O2 Plasma device platform | ||
2. Run the coating procedure for 1 minute | *2. Run the coating procedure for 1 minute | ||
3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)'' | *3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)'' | ||
Revision as of 09:13, 26 October 2006
Polydimethylsiloxane PDMS Prep
- 1. Add 10g PDMS and 1g curing agent to a weigh boat
- 2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles)
- 3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone
Spin Coating
- 1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat
- 2. Attach the selected mold to the spin coating machine
- 3. Pour PDMS slowly and evenly over the entire mold
- 4. Spin the mold for 30 seconds at 500rpm
- 5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C
- 6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage)
PDMS Surface Treatment via O2 Plasma
- 1. Place the PDMS channels "face up" on the O2 Plasma device platform
- 2. Run the coating procedure for 1 minute
- 3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)
