Penn State University 2006:Microchannel

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== Polydimethylsiloxane PDMS Prep ==

1. Add 10g PDMS and 1g curing agent to a weigh boat 2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles) 3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone


Spin Coating

1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat 2. Attach the selected mold to the spin coating machine 3. Pour PDMS slowly and evenly over the entire mold 4. Spin the mold for 30 seconds at 500rpm 5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C 6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage)


PDMS surface treatment via O2 Plasma

1. Place the PDMS channels "face up" on the O2 Plasma device platform 2. Run the coating procedure for 1 minute 3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)

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