Revision as of 16:04, 24 October 2006

Plate Reading

Time: variable

This is a kinetic assay for induction by HSL over a 107-magnitude concentration. Each construct is measured in triplicate.

Plates that are needed: 3+X shaker/growing, X fluorescence, where 2X is number of time points desired.

Condition parts on M9+0.1%YE+antibiotic minimal media plates
Inoculate 3 single colonies from these plates into M9+0.1%YE+antibiotic media overnight
Autoclave 96 well shaker/growing plates
Dilute cells down to OD600=0.1

For GFP, assay at 30ºC

@@ Line 6: / Line 6: @@
 This is a kinetic assay for induction by HSL over a 107-magnitude concentration.  Each construct is measured in triplicate.
+===<font color="red">Media/Reagents/Materials</font>===
 Plates that are needed: 3+X shaker/growing, X fluorescence, where 2X is number of time points desired.
+*M9+0.1%YE+antibiotic plates
+*210 mL M9+0.1%YE+antibiotic
+*96 well growing plates
+*96 well fluorescence plates
+*96 well clear bottom absorbance plates
-===<font color="red">Media/Reagents</font>===
+===<font color="orange">Pre</font>===
-M9+0.1%YE+antibiotic plates
+#Condition parts on M9+0.1%YE+antibiotic minimal media plates
-mL M9+0.1%YE+antibiotic
+#Inoculate 3 single colonies from these plates into M9+0.1%YE+antibiotic media overnight
-well growing plates
+#Autoclave 96 well shaker/growing plates
-well fluorescence plates
+#Dilute cells down to OD600=0.1
-well clear bottom absorbance plates
-Pre
+===<font color="blue">Plate Reading</font>===
-•	Condition parts on M9+0.1%YE+antibiotic minimal media plates
+#Do not put cells on ice!
-•	Inoculate 3 single colonies from these plates into M9+0.1%YE+antibiotic media overnight
-•	Autoclave 96 well shaker/growing plates
-•	Dilute cells down to OD600=0.1
-Plate Reading
-.  Do not put cells on ice!
 For GFP, assay at 30ºC
 ''