Penn State University 2006:lbplates: Difference between revisions
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6. Allow to solidify & store in bag in cold-room | 6. Allow to solidify & store in bag in cold-room | ||
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Revision as of 17:00, 24 October 2006
1LB Plates (per/L H2O)
1. Weigh and add to H2O in large Erlenmeyer: 10 g Bacto-tryptone 5 g Bacto-yeast extract 10 g NaCl 15 g Bacto-agar
2. Autoclave on slow exhaust cycle 25 min 3. Cool in tap-water-bath 10 min, then place in 45ºC water bath. (in sterile hood) 4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic. 5. Pour plates (~25 mL/plate) 6. Allow to solidify & store in bag in cold-room
