Pfu: Difference between revisions
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==Procurement== | ==Procurement== | ||
*You can buy Pfu for example at [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1311&ckt=1 Promega]. | *You can buy Pfu for example at [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1311&ckt=1 Promega] or [http://www.fermentas.com/catalog/modifyingenzymes/pfudnapolymer.htm Fermentas]. | ||
*NEB does for some reason not offer Pfu. | *NEB does for some reason not offer Pfu. | ||
Revision as of 10:56, 23 April 2008
Purpose
- Pfu is a thermostable DNA polymerase that can be used for PCR.
Procurement
Use
- Pfu has proof reading activity which makes it more accurate than for example Taq.
- Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.
