Pfu

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Revision as of 12:56, 23 April 2008 by Roy Ummels (Talk | contribs)
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Purpose

  • Pfu is a thermostable DNA polymerase that can be used for PCR.

Procurement

  • You can buy Pfu for example at Promega or Fermentas.
  • NEB does for some reason not offer Pfu.

Use

  • Pfu has proof reading activity which makes it more accurate than for example Taq.
  • Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.
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