http://www.openwetware.org/index.php?title=Phleomycin&feed=atom&action=history 2013-05-19T06:06:18Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=Phleomycin&diff=134048&oldid=prev 2007-07-26T17:47:49Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:47, 26 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 11:</td> <td colspan="2" class="diff-lineno">Line 11:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The best price I've found is through Invivogen</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The best price I've found is through Invivogen</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]] [[Category:Antibiotic]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-19 06:06:18 --> </table>

Reshma P. Shetty http://www.openwetware.org/index.php?title=Phleomycin&diff=11531&oldid=prev 2005-06-01T13:55:34Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:55, 1 June 2005</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The stock solution is <del class="diffchange diffchange-inline">10 </del>ug/mL (<del class="diffchange diffchange-inline">1000X</del>), is light blue and is stored at -20 C. It should be stable for 1 year.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The stock solution is <ins class="diffchange diffchange-inline">20 </ins>ug/mL (<ins class="diffchange diffchange-inline">2000X</ins>), is light blue and is stored at -20 C. It should be stable for 1 year.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&#160;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The best price I've found is through Invivogen</ins></div></td></tr> <!-- diff generator: internal 2013-05-19 06:06:18 --> </table>

Jgritton http://www.openwetware.org/index.php?title=Phleomycin&diff=759&oldid=prev 2005-05-12T15:41:20Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:41, 12 May 2005</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Used to select for </del>the ble <del class="diffchange diffchange-inline">marker in yeast.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Mode of Action==</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>&#160;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Binds and cleaves DNA</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Mechanism of Resistance==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The product of </ins>the <ins class="diffchange diffchange-inline">''Sh </ins>ble<ins class="diffchange diffchange-inline">'' gene binds to phleomycin and inhibits its ability to cleave DNA</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Usage==</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 06:06:18 --> </table>

Jgritton http://www.openwetware.org/index.php?title=Phleomycin&diff=497&oldid=prev 2005-05-06T17:38:42Z

<p></p> <p><b>New page</b></p><div>Used to select for the ble marker in yeast.<br /> <br /> After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.<br /> <br /> Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.<br /> <br /> The stock solution is 10 ug/mL (1000X), is light blue and is stored at -20 C. It should be stable for 1 year.</div>

Jgritton