Phosphate buffer - Revision history

Torsten Waldminghaus: added tag

2008-03-11T21:37:58Z

added tag

←Older revision		Revision as of 21:37, 11 March 2008
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-	[[Category:Material]]	+	[[Category:Material]] [[Category:Buffers]]

Dadrummond: /* Contact */

2007-06-20T14:59:47Z

Contact

←Older revision		Revision as of 14:59, 20 June 2007
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	*Any papers or websites that are relevant to the use of this material		*Any papers or websites that are relevant to the use of this material

-	~~==Contact==~~
-	*Who has experience using this material?

-
-	~~Add any other categories that may be relevant to your page. We use categories like tags.~~
	[[Category:Material]]		[[Category:Material]]

Dadrummond: /* 0.2 M monobasic stock */ monobasic formula

2007-06-20T14:59:09Z

0.2 M monobasic stock: monobasic formula

←Older revision		Revision as of 14:59, 20 June 2007
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	===0.2 M monobasic stock===		===0.2 M monobasic stock===
-	*15.6 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>~~HPO~~<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol), or 13.9 g of the anhydrous form	+	*15.6 g sodium phosphate monobasic dihydrate (NaH<sub>2</sub>PO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol), or 13.9 g of the anhydrous form
	*500 mL dH<sub>2</sub>O		*500 mL dH<sub>2</sub>O

Dadrummond: /* 0.2 M dibasic stock */

2007-06-20T14:58:48Z

0.2 M dibasic stock

←Older revision		Revision as of 14:58, 20 June 2007
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	===0.2 M dibasic stock===		===0.2 M dibasic stock===
-	*53.65 g sodium phosphate dibasic heptahydrate (Na<sub>2</sub>HPO<sub>4</sub>·7H<sub>2</sub>O, MW 268.07 g/mol) (or 28.4 g of the anhydrous form)	+	*53.65 g sodium phosphate dibasic heptahydrate (Na<sub>2</sub>HPO<sub>4</sub>·7H<sub>2</sub>O, MW 268.07 g/mol), or 28.4 g of the anhydrous form
	*1 L dH<sub>2</sub>O		*1 L dH<sub>2</sub>O

Dadrummond: /* 0.2 M monobasic stock */

2007-06-20T14:57:35Z

0.2 M monobasic stock

←Older revision		Revision as of 14:57, 20 June 2007
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	===0.2 M monobasic stock===		===0.2 M monobasic stock===
-	*15.6 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol, or 13.9 g of the anhydrous form)	+	*15.6 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol), or 13.9 g of the anhydrous form
	*500 mL dH<sub>2</sub>O		*500 mL dH<sub>2</sub>O

Dadrummond: /* 0.2 M monobasic stock */ fixed MW

2007-06-20T14:57:13Z

0.2 M monobasic stock: fixed MW

←Older revision		Revision as of 14:57, 20 June 2007
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	===0.2 M monobasic stock===		===0.2 M monobasic stock===
-	*13.9 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol)	+	*15.6 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol, or 13.9 g of the anhydrous form)
	*500 mL dH<sub>2</sub>O		*500 mL dH<sub>2</sub>O

Dadrummond: /* 0.2 M dibasic stock */

2007-06-20T14:55:22Z

0.2 M dibasic stock

←Older revision		Revision as of 14:55, 20 June 2007
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	===0.2 M dibasic stock===		===0.2 M dibasic stock===
-	53.65 g sodium phosphate dibasic heptahydrate (Na<sub>2</sub>HPO<sub>4</sub>·7H<sub>2</sub>O, MW 268.07 g/mol) (or 28.4 g of the anhydrous form)	+	*53.65 g sodium phosphate dibasic heptahydrate (Na<sub>2</sub>HPO<sub>4</sub>·7H<sub>2</sub>O, MW 268.07 g/mol) (or 28.4 g of the anhydrous form)
-	1 L dH<sub>2</sub>O	+	*1 L dH<sub>2</sub>O

	===0.1 M sodium phosphate buffer===		===0.1 M sodium phosphate buffer===

Dadrummond: /* 0.2 M monobasic stock */

2007-06-20T14:55:06Z

0.2 M monobasic stock

←Older revision		Revision as of 14:55, 20 June 2007
Line 6:		Line 6:

	===0.2 M monobasic stock===		===0.2 M monobasic stock===
-	13.9 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol)	+	*13.9 g sodium phosphate monobasic dihydrate (Na<sub>2</sub>HPO<sub>4</sub>·2H<sub>2</sub>O, MW 155.99 g/mol)
-	500 mL dH<sub>2</sub>O	+	*500 mL dH<sub>2</sub>O

	===0.2 M dibasic stock===		===0.2 M dibasic stock===

Dadrummond: new page

2007-06-20T14:54:37Z

new page

New page

==Purpose==
Phosphate buffer is a multi-purpose [http://en.wikipedia.org/wiki/Buffer_solution buffer] used for many molecular biology procedures.

==How to make phosphate buffer==
Source: Sörensen; in Hayat, 1986.

===0.2 M monobasic stock===
13.9 g sodium phosphate monobasic dihydrate (Na2HPO4·2H2O, MW 155.99 g/mol)
500 mL dH2O

===0.2 M dibasic stock===
53.65 g sodium phosphate dibasic heptahydrate (Na2HPO4·7H2O, MW 268.07 g/mol) (or 28.4 g of the anhydrous form)
1 L dH2O

===0.1 M sodium phosphate buffer===

To make 600mL 0.1 M sodium phosphate buffer, combine the following quantities of each stock and add dH2O to bring up to 600 mL:

<TABLE BORDER="1" WIDTH="25%" BORDER="0" CELLSPACING="0" CELLPADDING="0">
<TR>
<TD WIDTH="10%">monobasic (mL)</TD>
<TD WIDTH="10%">dibasic (mL)</TD>
<TD WIDTH="10%">pH</TD>
</TR>
<TR>
<TD WIDTH="10%">280.5</TD>
<TD WIDTH="10%">19.5</TD>
<TD WIDTH="10%">5.7</TD>
</TR>
<TR>
<TD WIDTH="10%">276</TD>
<TD WIDTH="10%">24</TD>
<TD WIDTH="10%">5.8</TD>
</TR>

<TR>
<TD WIDTH="10%">270</TD>
<TD WIDTH="10%"> 30</TD>
<TD WIDTH="10%">5.9</TD>
</TR>
<TR>
<TD WIDTH="10%">263.1 </TD>

<TD WIDTH="10%">36.9</TD>
<TD WIDTH="10%">6.0</TD>
</TR>
<TR>
<TD WIDTH="10%">255 </TD>
<TD WIDTH="10%">45</TD>
<TD WIDTH="10%">6.1</TD>

</TR>
<TR>
<TD WIDTH="10%">244.5 </TD>
<TD WIDTH="10%">55.5</TD>
<TD WIDTH="10%">6.2</TD>
</TR>
<TR>
<TD WIDTH="10%">232.5 </TD>

<TD WIDTH="10%">67.5</TD>
<TD WIDTH="10%">6.3</TD>
</TR>
<TR>
<TD WIDTH="10%">220.5 </TD>
<TD WIDTH="10%">79.5</TD>
<TD WIDTH="10%">6.4</TD>

</TR>
<TR>
<TD WIDTH="10%">205.5 </TD>
<TD WIDTH="10%">94.5</TD>
<TD WIDTH="10%">6.5</TD>
</TR>
<TR>
<TD WIDTH="10%">187.5 </TD>
<TD WIDTH="10%">112.5</TD>
<TD WIDTH="10%">6.6</TD>
</TR>
<TR>
<TD WIDTH="10%">169.5 </TD>
<TD WIDTH="10%">130.5</TD>
<TD WIDTH="10%">6.7</TD>

</TR>
<TR>
<TD WIDTH="10%">153 </TD>
<TD WIDTH="10%">147</TD>
<TD WIDTH="10%">6.8</TD>
</TR>
<TR>
<TD WIDTH="10%">135 </TD>
<TD WIDTH="10%">165</TD>
<TD WIDTH="10%">6.9</TD>
</TR>
<TR>
<TD WIDTH="10%">117 </TD>
<TD WIDTH="10%">183</TD>
<TD WIDTH="10%">7.0</TD>

</TR>
<TR>
<TD WIDTH="10%">99 </TD>
<TD WIDTH="10%">201</TD>
<TD WIDTH="10%">7.1</TD>
</TR>
<TR>
<TD WIDTH="10%">84 </TD>

<TD WIDTH="10%">216</TD>
<TD WIDTH="10%">7.2</TD>
</TR>
<TR>
<TD WIDTH="10%">69 </TD>
<TD WIDTH="10%">231</TD>
<TD WIDTH="10%">7.3</TD>

</TR>
<TR>
<TD WIDTH="10%">57 </TD>
<TD WIDTH="10%">243</TD>
<TD WIDTH="10%">7.4</TD>
</TR>
<TR>
<TD WIDTH="10%">48 </TD>

<TD WIDTH="10%">252</TD>
<TD WIDTH="10%">7.5</TD>
</TR>
<TR>
<TD WIDTH="10%">39 </TD>
<TD WIDTH="10%">261</TD>
<TD WIDTH="10%">7.6</TD>

</TR>
<TR>
<TD WIDTH="10%">31.5 </TD>
<TD WIDTH="10%">271.5</TD>
<TD WIDTH="10%">7.7</TD>
</TR>
<TR>
<TD WIDTH="10%">25.5 </TD>

<TD WIDTH="10%">274.5</TD>
<TD WIDTH="10%">7.8</TD>
</TR>
<TR>
<TD WIDTH="10%">21 </TD>
<TD WIDTH="10%">279</TD>
<TD WIDTH="10%">7.9</TD>

</TR>
<TR>
<TD WIDTH="10%">15.9 </TD>
<TD WIDTH="10%">284.1</TD>
<TD WIDTH="10%">8.0</TD>
</TR>
</TABLE>

==Use==

==Safety==
*Are there any safety considerations for this material?

==References==
*Any papers or websites that are relevant to the use of this material

==Contact==
*Who has experience using this material?

Add any other categories that may be relevant to your page. We use categories like tags.
[[Category:Material]]