Phusion
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| - | + | ==Description== | |
| - | + | Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity. | |
| - | + | ==Statistics about Phusion™ High-Fidelity DNA Polymerase== | |
| - | + | *10x processivity compared to Taq | |
| - | + | *50x fidelity compared to Taq | |
| - | + | *Creates blunt end | |
| - | + | *Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer | |
| - | + | *Non-displacing | |
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| + | ==References== | ||
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | *[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | ||
*[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual] | *[http://www.finnzymes.fi/products/pcr/phusion_datasheet_f530sl_1_3_low.pdf Manual] | ||
Revision as of 15:23, 13 September 2006
Description
Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Statistics about Phusion™ High-Fidelity DNA Polymerase
- 10x processivity compared to Taq
- 50x fidelity compared to Taq
- Creates blunt end
- Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
- Non-displacing
