What is it?
• a Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Stats about Phusion™ High-Fidelity DNA Polymerase
• 10x processivity compared to Taq
• 50x fidelity compared to Taq
• creates blunt end
• error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer