Picking Colonies

Picking Colonies (4/6) based on S. Boyarskiy's protocol:

Materials needed

• 5mL (Note: that's milli, not µ) pipette -- in the JCA lab, this can be found in cabinets in rear of room. Return after use.
• Use LB with antibiotic necessary for your experiment (Spec in our case for toxR plasmids)
• 1 test tube, labeled
• 1 bunsen burner (to sterilize pipette tip before drawing 5mL of antibiotic-LB, tube cap)
  • Do NOT breach the Z-axis of the burner's flame when grabbing materials on bench top/counter space.
• toothpick to pick colony.

Tips for picking colonies

• Pick an average colony (Not the biggest or smallest).
• Make sure it is not in contact with any other colonies.
• After putting 5mL of antibiotic-LB mixture into tube, lightly press toothpick into selected colony and drop into test tube.

Incubating picked colony in Warm Room (448A Stanley)

• Turn off shaker before placing tube into rack.
• Make sure to turn the shaker back on before you leave, don't F other's experiments up.
• Turn light to room off after you are done. (On wall in hallway)