Potassium phosphate solution: Difference between revisions
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===Ingredients=== | ===Ingredients=== | ||
For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are 0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole) | For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are: | ||
*0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole) | |||
*0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4, molecular weight=174.18 grams/mole). | |||
Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution through a 0.22 micron filter. | Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution through a 0.22 micron filter OR autoclave. | ||
Latest revision as of 08:46, 29 May 2012
Introduction and Purpose
For use in Terrific Broth this mixture should be diluted 1:10 in final mixture
Ingredients
For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are:
- 0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole)
- 0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4, molecular weight=174.18 grams/mole).
Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution through a 0.22 micron filter OR autoclave.
