Prather:HPLC: Difference between revisions
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*The method AMINEXBACKFLUSH.M can be used to automatically backflush the column | *The method AMINEXBACKFLUSH.M can be used to automatically backflush the column | ||
**When running supernatants or samples containing salts using this method, run a blank every 10-15 samples | **When running supernatants or samples containing salts using this method, run a blank every 10-15 samples | ||
**The method's run time is 45 min | |||
**This method should allow users to run > 15 samples without loss of resolution or the need to perform a manual backflush | |||
*A guard column has been purchased specifically for the Aminex column | *A guard column has been purchased specifically for the Aminex column | ||
**Please use the guard to protect the analytical column and extend its life | |||
**Install and remove the guard column and analytical column as a single unit | **Install and remove the guard column and analytical column as a single unit | ||
====Other Implications==== | |||
*Before installing your column, flush the entire HPLC system with water with no column installed to remove the previous user's mobile phase | |||
* | |||
==Maintenance Log== | ==Maintenance Log== | ||
Revision as of 10:03, 16 August 2007
Important Update
Transition to Single Column System
As of August 14, 2007 the HPLC is set up as a single column system to accomodate backflushing procedures for the Aminex column. This means that only one column can be installed at a time. The column connectors and flow direction are marked on the HPLC tubing.
With this new system configuration, ALL users will need to modify their methods as follows:
- Reconfigure the column/thermostat to 'Backflushing off' rather than simply 'Column 1' or 'Column 2'
- Set the thermostat for both sides of the heating block if your column straddles both of them.
Aminex Column Updates
- The method AMINEXBACKFLUSH.M can be used to automatically backflush the column
- When running supernatants or samples containing salts using this method, run a blank every 10-15 samples
- The method's run time is 45 min
- This method should allow users to run > 15 samples without loss of resolution or the need to perform a manual backflush
- A guard column has been purchased specifically for the Aminex column
- Please use the guard to protect the analytical column and extend its life
- Install and remove the guard column and analytical column as a single unit
Other Implications
- Before installing your column, flush the entire HPLC system with water with no column installed to remove the previous user's mobile phase
Maintenance Log
10Aug2007: Both pump seals (Agilent part# 5063-6589) were replaced after a steady leak from the pump head was detected. The seal wear-in procedure was followed, however instead of pumping isopropanol at 350 bar, a lower pressure of ~50-100 bar was used due to leaky fittings.
11Aug2007: Aminex column was cleaned and regenerated according to the manufacturers protocols.
Reserving Time on the HPLC
The HPLC in the Prather Lab is currently in high demand. We've set up an electronic calendar to ensure that everyone has enough time to use the instrument. The calendar is managed through Google calendar, and you can access the calendar to reserve the HPLC ahead of time by following the steps below:
- If you have a Google account, subscribe to the Prather Lab HPLC calendar by clicking on the button below.
<html><a target="_blank" href="http://www.google.com/calendar/render?cid=bcbp49couugtcksadk4j7kcat4%40group.calendar.google.com"><img src="http://www.google.com/calendar/images/ext/gc_button1_en.gif" border=0></a></html>
- If you don't have a Google account, you can sign up for one using an existing email address by clicking here.
- Once you have a Google account and have subscribed to the calendar, email dmbower@mit.edu with your Google username if you would like edit access. With edit access, you can use the calendar to reserve the HPLC.
HPLC Schedule
<html><iframe src="http://www.google.com/calendar/embed?src=bcbp49couugtcksadk4j7kcat4%40group.calendar.google.com&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="588"></iframe></html>
