Prather:Protocols: Difference between revisions
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*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | *[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | ||
**Growing ''E. coli'' in liquid culture and on solid media | |||
**DNA electrophoresis | |||
**Gel extraction | |||
**PCR | |||
**DNA digestion with restriction enzymes | |||
**DNA ligation reaction | |||
**Electrocompetent and chemical transformations | |||
**Preparation of freezer stocks | |||
*[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]] (As of 16Dec2006) | *[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]] (As of 16Dec2006) | ||
**Preparation of bacterial lysates by freeze-thaw | |||
**Bradford total protein assay | |||
**Boiling-lysis preparation of pDNA | |||
**Sample dialysis | |||
**Running a protein gel | |||
*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]] (As of 14Aug2007) | *[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]] (As of 14Aug2007) | ||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] | *[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] | ||
Revision as of 14:21, 16 August 2007
This page contains protocols and documents used by the Prather Lab at MIT.
- Basic Molecular Biology Protocols (As of 25Jun2007)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent and chemical transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (As of 16Dec2006)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
- Prather Lab New Student Guide (As of 14Aug2007)
- Good Laboratory Practice Handout
- Maintenance of Competent Cell Stocks
