Prather:Protocols

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*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)
 +
**Growing ''E. coli'' in liquid culture and on solid media
 +
**DNA electrophoresis
 +
**Gel extraction
 +
**PCR
 +
**DNA digestion with restriction enzymes
 +
**DNA ligation reaction
 +
**Electrocompetent and chemical transformations
 +
**Preparation of freezer stocks
 +
*[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]]  (As of 16Dec2006)
*[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]]  (As of 16Dec2006)
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**Preparation of bacterial lysates by freeze-thaw
 +
**Bradford total protein assay
 +
**Boiling-lysis preparation of pDNA
 +
**Sample dialysis
 +
**Running a protein gel
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*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]]  (As of 14Aug2007)
*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]]  (As of 14Aug2007)
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]]
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]]

Revision as of 17:21, 16 August 2007

This page contains protocols and documents used by the Prather Lab at MIT.

  • Basic Molecular Biology Protocols (As of 25Jun2007)
    • Growing E. coli in liquid culture and on solid media
    • DNA electrophoresis
    • Gel extraction
    • PCR
    • DNA digestion with restriction enzymes
    • DNA ligation reaction
    • Electrocompetent and chemical transformations
    • Preparation of freezer stocks
  • Advanced Molecular Biology Protocols (As of 16Dec2006)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel










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