Prather:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
This page contains protocols and documents used by the Prather Lab at MIT.
+
===Prather Lab Protocols and Useful Documents===
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)

Revision as of 17:22, 16 August 2007

Prather Lab Protocols and Useful Documents

  • Basic Molecular Biology Protocols (As of 25Jun2007)
    • Growing E. coli in liquid culture and on solid media
    • DNA electrophoresis
    • Gel extraction
    • PCR
    • DNA digestion with restriction enzymes
    • DNA ligation reaction
    • Electrocompetent and chemical transformations
    • Preparation of freezer stocks
  • Advanced Molecular Biology Protocols (As of 16Dec2006)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel










Back to Prather Lab Home

Personal tools