Prather:Protocols

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(Prather Lab Protocols and Useful Documents)
(Prather Lab Protocols and Useful Documents)
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===Prather Lab Protocols and Useful Documents===
===Prather Lab Protocols and Useful Documents===
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*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]]  (As of 14Aug2007)
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*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]]
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007)
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**Running a protein gel
**Running a protein gel
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*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]]  (As of 14Aug2007)
 
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*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]]
 
*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]]
*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]]
*[[media:Undergraduate_Student_Evaluation_Form.doc|Undergraduate Student Evaluation Form]]‎  
*[[media:Undergraduate_Student_Evaluation_Form.doc|Undergraduate Student Evaluation Form]]‎  

Revision as of 13:31, 24 August 2007

Prather Lab Protocols and Useful Documents

  • Basic Molecular Biology Protocols (As of 25Jun2007)
    • Growing E. coli in liquid culture and on solid media
    • DNA electrophoresis
    • Gel extraction
    • PCR
    • DNA digestion with restriction enzymes
    • DNA ligation reaction
    • Electrocompetent and chemical transformations
    • Preparation of freezer stocks
  • Advanced Molecular Biology Protocols (As of 16Dec2006)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel









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