Prather:Protocols: Difference between revisions
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*[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]] (As of 14Aug2007) | *[[media:New_Member_Guide_14Aug2007.pdf|Prather Lab New Student Guide]] (As of 14Aug2007) | ||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] | *[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (As of 15Mar2007) | ||
*[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | *[[media:Prather Lab Experimental Protocols 25Jun2007.doc|Basic Molecular Biology Protocols]] (As of 25Jun2007) | ||
**Growing ''E. coli'' in liquid culture and on solid media | **Growing ''E. coli'' in liquid culture and on solid media | ||
Revision as of 10:33, 24 August 2007
Prather Lab Protocols and Useful Documents
- Prather Lab New Student Guide (As of 14Aug2007)
- Good Laboratory Practice Handout (As of 15Mar2007)
- Basic Molecular Biology Protocols (As of 25Jun2007)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent and chemical transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (As of 16Dec2006)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
