Prather:Protocols: Difference between revisions
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===Prather Lab Protocols=== | ===Prather Lab Protocols=== | ||
*[[media: | *[[media:New_Member_Guide_25Jan2009.pdf|Prather Lab New Student Guide]] (Updated Jan. 2009) | ||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (As of 15Mar2007) | *[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (As of 15Mar2007) | ||
*[[media: | *[[media:Prather_Lab_Experimental_Protocols_25Jan2009.pdf|Basic Molecular Biology Protocols]] (Updated Jan. 2009) | ||
**Growing ''E. coli'' in liquid culture and on solid media | **Growing ''E. coli'' in liquid culture and on solid media | ||
**DNA electrophoresis | **DNA electrophoresis | ||
Revision as of 12:41, 4 February 2009
Prather Lab Protocols
- Prather Lab New Student Guide (Updated Jan. 2009)
- Good Laboratory Practice Handout (As of 15Mar2007)
- Basic Molecular Biology Protocols (Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent and chemical transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (As of 16Dec2006)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
