Prather:Protocols: Difference between revisions
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**DNA digestion with restriction enzymes | **DNA digestion with restriction enzymes | ||
**DNA ligation reaction | **DNA ligation reaction | ||
**Electrocompetent | **Electrocompetent transformations | ||
**Preparation of freezer stocks | **Preparation of freezer stocks | ||
Revision as of 12:42, 4 February 2009
Prather Lab Protocols
- Prather Lab New Student Guide (Updated Jan. 2009)
- Good Laboratory Practice Handout (As of 15Mar2007)
- Basic Molecular Biology Protocols (Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (As of 16Dec2006)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel