Prather:Protocols: Difference between revisions
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**Preparation of freezer stocks | **Preparation of freezer stocks | ||
*[[media: | *[[media:Advanced_Protocols_2009.pdf|Advanced Molecular Biology Protocols]] (PDF, Updated Jan. 2009) | ||
**Preparation of bacterial lysates by freeze-thaw | **Preparation of bacterial lysates by freeze-thaw | ||
**Bradford total protein assay | **Bradford total protein assay | ||
Revision as of 13:31, 4 February 2009
Prather Lab Protocols
- Prather Lab New Student Guide (PDF, Updated Jan. 2009)
- Good Laboratory Practice Handout (PDF, Updated 15Mar2007)
- Basic Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
