Prather:Protocols
From OpenWetWare
(Difference between revisions)
| Line 21: | Line 21: | ||
*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]] | *[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]] | ||
| - | *[[Prather:Gibson Chew Back and Anneal | + | *[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]] |
*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]] (PDF, Updated Feb. 2009) | *[[media:P_putida_protocols.pdf|''P. putida'' Protocols]] (PDF, Updated Feb. 2009) | ||
---- | ---- | ||
[[Prather | Back to Prather Lab Home]] | [[Prather | Back to Prather Lab Home]] | ||
Revision as of 18:48, 8 August 2010
Prather Lab Protocols
- Prather Lab New Student Guide (PDF, Updated Jan. 2009)
- Good Laboratory Practice Handout (PDF, Updated 15Mar2007)
- Basic Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
- Maintenance of Competent Cell Stocks
- Gibson Chew Back and Anneal Isothermal Assembly
- P. putida Protocols (PDF, Updated Feb. 2009)
