Prather:Protocols

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(Prather Lab Protocols)
(Prather Lab Protocols)
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**Running a protein gel
**Running a protein gel
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*[[Prather:DH10B Stocks | Maintenance of Competent Cell Stocks]]
 
*[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]]
*[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]]
*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]]  (PDF, Updated Feb. 2009)
*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]]  (PDF, Updated Feb. 2009)

Revision as of 17:52, 1 March 2011

Prather Lab Protocols

  • Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel

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