Prather:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (7 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
===Prather Lab Protocols=== | ===Prather Lab Protocols=== | ||
*[[media: | *[[media:New_Member_Guide_-_6June11.pdf|Prather Lab New Student Guide]] (PDF, Updated June 2011) | ||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007) | *[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007) | ||
*[[media:Prather_Lab_Experimental_Protocols_25Jan09.pdf|Basic Molecular Biology Protocols]] (PDF, Updated Jan. 2009) | *[[media:Prather_Lab_Experimental_Protocols_25Jan09.pdf|Basic Molecular Biology Protocols]] (PDF, Updated Jan. 2009) | ||
| Line 20: | Line 20: | ||
**Running a protein gel | **Running a protein gel | ||
*[[Prather: | *[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]] | ||
*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]] (PDF, Updated Feb. 2009) | *[[media:P_putida_protocols.pdf|''P. putida'' Protocols]] (PDF, Updated Feb. 2009) | ||
---- | ---- | ||
[[Prather | Back to Prather Lab Home]] | [[Prather | Back to Prather Lab Home]] | ||
Revision as of 09:26, 6 June 2011
Prather Lab Protocols
- Prather Lab New Student Guide (PDF, Updated June 2011)
- Good Laboratory Practice Handout (PDF, Updated 15Mar2007)
- Basic Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent transformations
- Preparation of freezer stocks
- Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
- Gibson Chew Back and Anneal Isothermal Assembly
- P. putida Protocols (PDF, Updated Feb. 2009)
