Prather:Protocols: Difference between revisions

Latest revision as of 11:41, 7 January 2014

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-This page will contain protocols commonly used by the Prather Lab.
+===Prather Lab Protocols===
-*[[media:Basic_Experimental_Protocols.doc|Basic Molecular Biology Protocols]] (Version 12-17-06)
-*[[media:Advanced_Protocols.doc|Advanced Molecular Biology Protocols]]  (Version 12-16-06)
+*[[media:New_Member_Guide_-_6June11.pdf‎|Prather Lab New Student Guide]]  (PDF, Updated June 2011)
+*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007)
+*[[media:Molecular_Cloning_Protocols_6Jan14.doc|Molecular Biology Protocols]] (PDF, Updated Jan. 2009)
+**Growing ''E. coli'' in liquid culture and on solid media
+**DNA electrophoresis
+**Gel extraction
+**PCR
+**DNA digestion with restriction enzymes
+**DNA ligation reaction
+**Electrocompetent transformations
+**Preparation of freezer stocks
+**Plasmid miniprepping
+**PIPE cloning
+*[[media:Advanced_Protocols_2009.pdf|Advanced Molecular Biology Protocols]]  (PDF, Updated Jan. 2009)
+**Preparation of bacterial lysates by freeze-thaw
+**Bradford total protein assay
+**Boiling-lysis preparation of pDNA
+**Sample dialysis
+**Running a protein gel
+*[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]]
+*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]]  (PDF, Updated Feb. 2009)
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