Prather:Protocols: Difference between revisions
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===Prather Lab Protocols=== | ===Prather Lab Protocols=== | ||
*[[media: | *[[media:New_Member_Guide_-_6June11.pdf|Prather Lab New Student Guide]] (PDF, Updated June 2011) | ||
*[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] ( | *[[media:Good_Laboratory_Practice_Handout.pdf|Good Laboratory Practice Handout]] (PDF, Updated 15Mar2007) | ||
*[[media: | *[[media:Molecular_Cloning_Protocols_6Jan14.doc|Molecular Biology Protocols]] (PDF, Updated Jan. 2009) | ||
**Growing ''E. coli'' in liquid culture and on solid media | **Growing ''E. coli'' in liquid culture and on solid media | ||
**DNA electrophoresis | **DNA electrophoresis | ||
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**DNA digestion with restriction enzymes | **DNA digestion with restriction enzymes | ||
**DNA ligation reaction | **DNA ligation reaction | ||
**Electrocompetent | **Electrocompetent transformations | ||
**Preparation of freezer stocks | **Preparation of freezer stocks | ||
**Plasmid miniprepping | |||
**PIPE cloning | |||
*[[media: | *[[media:Advanced_Protocols_2009.pdf|Advanced Molecular Biology Protocols]] (PDF, Updated Jan. 2009) | ||
**Preparation of bacterial lysates by freeze-thaw | **Preparation of bacterial lysates by freeze-thaw | ||
**Bradford total protein assay | **Bradford total protein assay | ||
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**Running a protein gel | **Running a protein gel | ||
*[[Prather: | *[[Prather:Gibson CBA| Gibson Chew Back and Anneal Isothermal Assembly]] | ||
*[[media:P_putida_protocols.pdf|''P. putida'' Protocols]] (PDF, Updated Feb. 2009) | |||
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[[Prather | Back to Prather Lab Home]] | [[Prather | Back to Prather Lab Home]] | ||
Latest revision as of 11:41, 7 January 2014
Prather Lab Protocols
- Prather Lab New Student Guide (PDF, Updated June 2011)
- Good Laboratory Practice Handout (PDF, Updated 15Mar2007)
- Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Growing E. coli in liquid culture and on solid media
- DNA electrophoresis
- Gel extraction
- PCR
- DNA digestion with restriction enzymes
- DNA ligation reaction
- Electrocompetent transformations
- Preparation of freezer stocks
- Plasmid miniprepping
- PIPE cloning
- Advanced Molecular Biology Protocols (PDF, Updated Jan. 2009)
- Preparation of bacterial lysates by freeze-thaw
- Bradford total protein assay
- Boiling-lysis preparation of pDNA
- Sample dialysis
- Running a protein gel
- Gibson Chew Back and Anneal Isothermal Assembly
- P. putida Protocols (PDF, Updated Feb. 2009)
