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This page contains protocols and documents used by the Prather Lab at MIT.

  • Basic Molecular Biology Protocols (As of 25Jun2007)
    • Growing E. coli in liquid culture and on solid media
    • DNA electrophoresis
    • Gel extraction
    • PCR
    • DNA digestion with restriction enzymes
    • DNA ligation reaction
    • Electrocompetent and chemical transformations
    • Preparation of freezer stocks
  • Advanced Molecular Biology Protocols (As of 16Dec2006)
    • Preparation of bacterial lysates by freeze-thaw
    • Bradford total protein assay
    • Boiling-lysis preparation of pDNA
    • Sample dialysis
    • Running a protein gel

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