Prather:Protocols

This page contains protocols and documents used by the Prather Lab at MIT.

• Basic Molecular Biology Protocols (As of 25Jun2007)
  • Growing E. coli in liquid culture and on solid media
  • DNA electrophoresis
  • Gel extraction
  • PCR
  • DNA digestion with restriction enzymes
  • DNA ligation reaction
  • Electrocompetent and chemical transformations
  • Preparation of freezer stocks

• Advanced Molecular Biology Protocols (As of 16Dec2006)
  • Preparation of bacterial lysates by freeze-thaw
  • Bradford total protein assay
  • Boiling-lysis preparation of pDNA
  • Sample dialysis
  • Running a protein gel

• Prather Lab New Student Guide (As of 14Aug2007)
• Good Laboratory Practice Handout
• Maintenance of Competent Cell Stocks

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