Prather:Protocols

Prather Lab Protocols

• Prather Lab New Student Guide (Updated Jan. 2009)
• Good Laboratory Practice Handout (As of 15Mar2007)
• Basic Molecular Biology Protocols (Updated Jan. 2009)
  • Growing E. coli in liquid culture and on solid media
  • DNA electrophoresis
  • Gel extraction
  • PCR
  • DNA digestion with restriction enzymes
  • DNA ligation reaction
  • Electrocompetent and chemical transformations
  • Preparation of freezer stocks

• Advanced Molecular Biology Protocols (As of 16Dec2006)
  • Preparation of bacterial lysates by freeze-thaw
  • Bradford total protein assay
  • Boiling-lysis preparation of pDNA
  • Sample dialysis
  • Running a protein gel

• Maintenance of Competent Cell Stocks

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