PrbbBB:Internal: Difference between revisions
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== BioBricks Format == | == BioBricks Format == | ||
The BioBricks standard defines a fixed [http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix prefix and suffix] sequence for wrapping DNA fragments. Prefix and Suffix each contain 2 different restriction sites which are used for the [http://partsregistry.org/Assembly:Standard_assemblystandard assembly reaction] (of which we use a quicker [[Synthetic_Biology:BioBricks/3A_assembly | alternative]). In order to construct your own BioBricks, you thus have to introduce these standard flanks ''and'' ensure that there are no BioBrick restriction sites within your fragment. | The BioBricks standard defines a fixed [http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix prefix and suffix] sequence for wrapping DNA fragments. Prefix and Suffix each contain 2 different restriction sites which are used for the [http://partsregistry.org/Assembly:Standard_assemblystandard assembly reaction] (of which we use a quicker [[Synthetic_Biology:BioBricks/3A_assembly | alternative]]). In order to construct your own BioBricks, you thus have to introduce these standard flanks ''and'' ensure that there are no BioBrick restriction sites within your fragment. | ||
Unfortunately, this standard BioBricks format does not allow for the creation of protein fusions. | Unfortunately, this standard BioBricks format does not allow for the creation of protein fusions. | ||
Revision as of 08:32, 15 June 2008
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BioBricks Collection
Our local "Biobricks" are currently stored in freezer boxes in the Serrano and Isalan labs. We generally try to have, for each, a cell stock at -80 and a DNA stock at -20. But bear with us, we are still sorting out some of the details and are very open to suggestions.
The plates with last year's MIT registry collection are available in the Serrano lab. The new collection has not arrived yet.
Our samples are tracked in our local "BrickIt" web repository:
(Currently, this web site is only visible from within the CRG network.)
But you are welcome to enter your BioBricks even before you have them -- this can be quite helpful for planning your experiments.
Contact Raik for a brief introduction into the BrickIt web site!
BioBricks Format
The BioBricks standard defines a fixed prefix and suffix sequence for wrapping DNA fragments. Prefix and Suffix each contain 2 different restriction sites which are used for the assembly reaction (of which we use a quicker alternative). In order to construct your own BioBricks, you thus have to introduce these standard flanks and ensure that there are no BioBrick restriction sites within your fragment.
Unfortunately, this standard BioBricks format does not allow for the creation of protein fusions.
