PrbbBB:Protocols: Difference between revisions
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We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel. | We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel. | ||
* [[Prbbbb:vector_pcr|prepare vector backbone for assembly]] ( | * [[Prbbbb:vector_pcr|prepare vector backbone for assembly]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format]) | ||
* [[Prbbbb:fusion_assembly_v1|Assembly protocol for BioBricks on 96 well plate]] ( | * [[Prbbbb:fusion_assembly_v1|Assembly protocol for BioBricks on 96 well plate]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format]) | ||
* [[PrbbBB:colony pcr v1|Colony PCR screening of many assemblies in parallel (on 96 well plate)]] | * [[PrbbBB:colony pcr v1|Colony PCR screening of many assemblies in parallel (on 96 well plate)]] | ||
* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] ( | * [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format]) | ||
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]] | * [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]] | ||
==Protein Expression== | ==Protein Expression== | ||
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | * [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]] | ||
Revision as of 04:09, 6 August 2009
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<td width=120><a href="PrbbBB">Home</td>
<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Members">Members</td>
<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Protocols">Protocols</td>
<td width=120><a href="http://openwetware.org/wiki/PrbbBB:Internal">Internal</td> <td width=120><a href="http://openwetware.org/wiki/PrbbBB:Contact">Contact</td>
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We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
- prepare vector backbone for assembly (RFC 25 Fusion format)
- Assembly protocol for BioBricks on 96 well plate (RFC 25 Fusion format)
- Colony PCR screening of many assemblies in parallel (on 96 well plate)
- Ligation-free Biobrick Construction by PCR/recombination (RFC 25 Fusion format)
- DNA measurement on plates
