PrbbBB:Protocols

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(Biobrick assembly & related)
Current revision (09:11, 8 August 2010) (view source)
 
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==Biobrick assembly & related==
==Biobrick assembly & related==
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* [[Prbbbb:vector_pcr|prepare vector backbone for assembly (Fusion format)]]
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We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
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* [[Prbbbb:fusion_assembly_v1|Assembly protocol for Fusion BioBricks]]
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* [[Prbbbb:fusion_biobrick_construction_v1|ligation-free Fusion Biobrick Construction by PCR/recombination]]
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* [[Prbbbb:vector_pcr|prepare vector backbone for assembly]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:fusion_assembly_v1|Assembly protocol for BioBricks on 96 well plate]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[PrbbBB:colony pcr v1|Colony PCR screening of many assemblies in parallel (on 96 well plate)]]
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
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* [[PrbbBB:Oligo_Annealing|Annealing of complementary long Oligos]]
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==Protein Expression==
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* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
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* [[Prbbbb:large_scale_expression_v1|Large-scale expression in BL21]]
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* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]]
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* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
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==Protein measurements==
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* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]]
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[[Category:Protocol]]

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Biobrick assembly & related

We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.

Protein Expression

Protein measurements

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