PrbbBB:Protocols

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(Biobrick assembly & related)
Current revision (09:11, 8 August 2010) (view source)
 
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We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.
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* [[Prbbbb:vector_pcr|prepare vector backbone for assembly]] (Freiburg [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:vector_pcr|prepare vector backbone for assembly]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:fusion_assembly_v1|Assembly protocol for BioBricks on 96 well plate]] (Freiburg [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:fusion_assembly_v1|Assembly protocol for BioBricks on 96 well plate]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
* [[PrbbBB:colony pcr v1|Colony PCR screening of many assemblies in parallel (on 96 well plate)]]
* [[PrbbBB:colony pcr v1|Colony PCR screening of many assemblies in parallel (on 96 well plate)]]
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (Freiburg [http://partsregistry.org/Help:Freiburg_standard Fusion format])
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
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* [[PrbbBB:Oligo_Annealing|Annealing of complementary long Oligos]]
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==Protein Expression==
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* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
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* [[Prbbbb:large_scale_expression_v1|Large-scale expression in BL21]]
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* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]]
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* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
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==Protein measurements==
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* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]]
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[[Category:Protocol]]

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Biobrick assembly & related

We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.

Protein Expression

Protein measurements

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