PrbbBB:Protocols

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(Protein Expression)
Current revision (08:11, 8 August 2010) (view source)
 
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* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
* [[Prbbbb:fusion_biobrick_construction_v1|Ligation-free Biobrick Construction by PCR/recombination]] (RFC 25 [http://partsregistry.org/Help:Freiburg_standard Fusion format])
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
* [[Prbbbb:dna_measurement_on_plate_v1|DNA measurement on plates]]
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* [[PrbbBB:Oligo_Annealing|Annealing of complementary long Oligos]]
==Protein Expression==
==Protein Expression==
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
* [[Prbbbb:small_scale_expression_v1|Small-scale expression in BL21]]
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* [[Prbbbb:large_scale_expression_v1|Large-scale expression in BL21]]
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* [[Prbbbb:inclusion_body_solubilization_v1|Solubilization of E. coli inclusion bodies]]
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
* [[Prbbbb:Coomassi_Ethanol_stain|Coomassi protein stain (Ethanol-based)]]
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==Protein measurements==
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* [[Prbbbb:in_vitro_FRET_FRB_FKBP_v1|''in-vitro'' FRET measurements]]
[[Category:Protocol]]
[[Category:Protocol]]

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Home Members Protocols Internal Contact

Biobrick assembly & related

We have streamlined the standard Biobrick assembly protocols. Our "Barcelona set" of protocols is optimized for performing many assemblies in parallel.

Protein Expression

Protein measurements

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