PrbbBB:colony pcr v1
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Overview
Materials
- 96-well PCR plate
- AmpliTaq DNA Polymerase 5 U/µl (Roche)
- AmpliTaq Buffer 10 x
- dNTP mix 10mM each nucleotide
- ddH2O
- primers:
Procedure
I PCR reaction
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II Agarose Gel
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
raik: no comment
References
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